Detailed information of TA system TA208918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2485..3075
• Replicon: plasmid pAV_175-4
• Accession: NZ_CP078030
• Organism: Acinetobacter variabilis strain AV_175

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N8YGE2
• Locus tag: EGK58_RS16610
• Protein ID: WP_004787583.1
• Coordinates: 2779..3075 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EGK58_RS16605
• Protein ID: WP_004787581.1
• Coordinates: 2485..2775 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGK58_RS16600 (EGK58_016600)	1595..1774	+	180	WP_125279691.1	hypothetical protein	-
EGK58_RS16605 (EGK58_016605)	2485..2775	-	291	WP_004787581.1	putative addiction module antidote protein	Antitoxin
EGK58_RS16610 (EGK58_016610)	2779..3075	-	297	WP_004787583.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGK58_RS16615 (EGK58_016615)	3399..4040	-	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
EGK58_RS16620 (EGK58_016620)	4106..5293	+	1188	WP_125279692.1	Tet(A)/Tet(B)/Tet(C) family tetracycline efflux MFS transporter	-
EGK58_RS16625 (EGK58_016625)	5519..6130	+	612	WP_015060246.1	recombinase family protein	-
EGK58_RS16630 (EGK58_016630)	6320..7204	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39) / mph(E) / msr(E)	-	1..19843	19843
	-	inside	IScluster/Tn	tet(39) / mph(E) / msr(E)	-	4172..12593	8421

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Uncharacterized protein HI1420 (9-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11598.27 Da
• Isoelectric Point: 6.4770

>T208918 WP_004787583.1 NZ_CP078030:c3075-2779 [Acinetobacter variabilis]
MTEIKRLPEFDEWLDSIKDSMTRLKLNRRLDKVQRGNWGDVKHLQEGVWEMREFFGPGWRMYYIKHGDVIIVMLGGGDKS
TQQKDIERAVDLSKSLED

Nucleotide

• Length: 297 bp

>T208918 NZ_CP103756:176589-176894 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10485.15 Da
• Isoelectric Point: 4.8096

>AT208918 WP_004787581.1 NZ_CP078030:c2775-2485 [Acinetobacter variabilis]
MVKIADLPSFDMAEALKSEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQNA

Nucleotide

• Length: 291 bp

>AT208918 NZ_CP103756:176369-176587 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A6C0XPU5