Detailed information of TA system TA208915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 24948..25532
• Replicon: plasmid pAV_175-2
• Accession: NZ_CP078028
• Organism: Acinetobacter variabilis strain AV_175

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: EGK58_RS15550
• Protein ID: WP_000286964.1
• Coordinates: 24948..25250 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: EGK58_RS15555
• Protein ID: WP_000985610.1
• Coordinates: 25251..25532 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGK58_RS15525 (EGK58_015525)	20541..21512	-	972	WP_125279862.1	helix-turn-helix domain-containing protein	-
EGK58_RS15530 (EGK58_015530)	21645..22529	+	885	WP_125279861.1	MBL fold metallo-hydrolase	-
EGK58_RS15535 (EGK58_015535)	22613..23155	+	543	WP_125279860.1	cysteine hydrolase family protein	-
EGK58_RS15540 (EGK58_015540)	23312..23857	-	546	WP_005176794.1	XRE family transcriptional regulator	-
EGK58_RS15545 (EGK58_015545)	23950..24828	+	879	WP_213071249.1	DMT family transporter	-
EGK58_RS15550 (EGK58_015550)	24948..25250	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGK58_RS15555 (EGK58_015555)	25251..25532	+	282	WP_000985610.1	putative addiction module antidote protein	Antitoxin
EGK58_RS15560 (EGK58_015560)	25624..25965	+	342	WP_104472901.1	hypothetical protein	-
EGK58_RS15565 (EGK58_015565)	26090..26521	-	432	WP_005108698.1	MarR family transcriptional regulator	-
EGK58_RS15570 (EGK58_015570)	26528..26956	-	429	WP_005108699.1	organic hydroperoxide resistance protein	-
EGK58_RS15575 (EGK58_015575)	27432..28277	+	846	WP_125279916.1	hypothetical protein	-
EGK58_RS15580 (EGK58_015580)	28489..29823	-	1335	WP_219014846.1	ISNCY family transposase	-
EGK58_RS15585 (EGK58_015585)	30159..30233	+	75	WP_075107140.1	pyrroloquinoline quinone precursor peptide PqqA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..196965	196965
	-	flank	IS/Tn	-	-	28489..29823	1334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T208915 WP_000286964.1 NZ_CP078028:24948-25250 [Acinetobacter variabilis]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T208915 NZ_CP103755:c5085443-5085132 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT208915 WP_000985610.1 NZ_CP078028:25251-25532 [Acinetobacter variabilis]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT208915 NZ_CP103755:c5085131-5084841 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0A8TV94