Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT-BrnA |
| Location | 172650..173238 | Replicon | plasmid pAJ_351-2 |
| Accession | NZ_CP078019 | ||
| Organism | Acinetobacter junii strain AJ_351 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | N8SM64 |
| Locus tag | EGT69_RS17135 | Protein ID | WP_000438825.1 |
| Coordinates | 172650..172937 (+) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | D3JXQ4 |
| Locus tag | EGT69_RS17140 | Protein ID | WP_004728120.1 |
| Coordinates | 172924..173238 (+) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EGT69_RS17115 (EGT69_017115) | 168830..170022 | + | 1193 | WP_085955245.1 | IS3-like element ISKpn18 family transposase | - |
| EGT69_RS17120 (EGT69_017120) | 170141..170755 | + | 615 | WP_125281634.1 | recombinase family protein | - |
| EGT69_RS17125 (EGT69_017125) | 170821..171624 | + | 804 | WP_125281635.1 | aminoglycoside O-phosphotransferase APH(3'')-Ib | - |
| EGT69_RS17130 (EGT69_017130) | 171624..172460 | + | 837 | WP_125281636.1 | APH(6)-I family aminoglycoside O-phosphotransferase | - |
| EGT69_RS17135 (EGT69_017135) | 172650..172937 | + | 288 | WP_000438825.1 | BrnT family toxin | Toxin |
| EGT69_RS17140 (EGT69_017140) | 172924..173238 | + | 315 | WP_004728120.1 | BrnA antitoxin family protein | Antitoxin |
| EGT69_RS17145 (EGT69_017145) | 173327..173857 | - | 531 | WP_000172759.1 | hypothetical protein | - |
| EGT69_RS17150 (EGT69_017150) | 174006..174308 | + | 303 | WP_000286964.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| EGT69_RS17155 (EGT69_017155) | 174309..174590 | + | 282 | WP_000985609.1 | putative addiction module antidote protein | - |
| EGT69_RS17160 (EGT69_017160) | 174818..175983 | + | 1166 | WP_085944217.1 | IS3-like element ISAba22 family transposase | - |
| EGT69_RS17165 (EGT69_017165) | 176013..176366 | - | 354 | WP_228738248.1 | SMI1/KNR4 family protein | - |
| EGT69_RS17170 (EGT69_017170) | 176506..176826 | + | 321 | WP_000897307.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| EGT69_RS17175 (EGT69_017175) | 176819..177091 | + | 273 | WP_000369781.1 | NadS family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | tet(X3) / sul2 / aph(3'')-Ib / aph(6)-Id / msr(E) / mph(E) / blaNDM-1 / ARR-3 / qacE / sul1 / aph(3')-VIa / blaOXA-58 | - | 1..332451 | 332451 | |
| - | inside | IScluster/Tn | aph(3'')-Ib / aph(6)-Id / msr(E) / mph(E) | - | 158384..179999 | 21615 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11304.70 Da Isoelectric Point: 5.6205
>T208910 WP_000438825.1 NZ_CP078019:172650-172937 [Acinetobacter junii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
Download Length: 288 bp
>T208910 NZ_CP103755:c4365614-4365327 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA
Antitoxin
Download Length: 105 a.a. Molecular weight: 12200.86 Da Isoelectric Point: 8.4628
>AT208910 WP_004728120.1 NZ_CP078019:172924-173238 [Acinetobacter junii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
Download Length: 315 bp
>AT208910 NZ_CP103755:c4365340-4365038 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TVQ4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TRI9 |