Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 2175645..2176120 Replicon chromosome
Accession NZ_CP078017
Organism Alcaligenes aquatilis strain 393

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag EGK70_RS10090 Protein ID WP_125276803.1
Coordinates 2175645..2175971 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag EGK70_RS10095 Protein ID WP_234354429.1
Coordinates 2175968..2176120 (-) Length 51 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGK70_RS10080 (EGK70_010080) 2170767..2174069 + 3303 WP_125276805.1 DEAD/DEAH box helicase family protein -
EGK70_RS10085 (EGK70_010085) 2174279..2175190 - 912 WP_125276804.1 hypothetical protein -
EGK70_RS10090 (EGK70_010090) 2175645..2175971 - 327 WP_125276803.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EGK70_RS10095 (EGK70_010095) 2175968..2176120 - 153 WP_234354429.1 DUF3018 family protein Antitoxin
EGK70_RS10100 (EGK70_010100) 2176352..2176723 - 372 WP_125276802.1 hypothetical protein -
EGK70_RS10105 (EGK70_010105) 2176876..2177682 + 807 WP_125276912.1 SprT-like domain-containing protein -
EGK70_RS10110 (EGK70_010110) 2177775..2178254 + 480 WP_125276801.1 PH domain-containing protein -
EGK70_RS10115 (EGK70_010115) 2178276..2179031 + 756 WP_125276800.1 DsbC family protein -
EGK70_RS10120 (EGK70_010120) 2179044..2179643 + 600 WP_125276799.1 hypothetical protein -
EGK70_RS10125 (EGK70_010125) 2179655..2180545 + 891 WP_125276798.1 hypothetical protein -
EGK70_RS10130 (EGK70_010130) 2180648..2180968 + 321 WP_125276797.1 H-NS histone family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2175408..2204924 29516


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-105)

Antitoxin

Antitoxin MazE-like

(1-48)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11613.65 Da        Isoelectric Point: 9.2533

>T208906 WP_125276803.1 NZ_CP078017:c2175971-2175645 [Alcaligenes aquatilis]
MRRGDLVTVAMQGDFGKPRPALVIQADLFAAHSSVTVLPVTSTIVNAPLLRISIQPDLENGLQKPSQIMIDKALTVKRDK
IGQTFGSVSADTLVEIERCLAVFLGIAK

Download         Length: 327 bp

>T208906 NZ_CP103755:c3774898-3774596 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 51 a.a.        Molecular weight: 5759.38 Da        Isoelectric Point: 4.2975

>AT208906 WP_234354429.1 NZ_CP078017:c2176120-2175968 [Alcaligenes aquatilis]
VQLWVPDTRRPDFAAECRRQALRVAKADKADVDTEHFMDEALADLDGWTE

Download         Length: 153 bp

>AT208906 NZ_CP103755:c3774560-3774219 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References