Detailed information of TA system TA208890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 1551585..1552722
• Replicon: chromosome
• Accession: NZ_CP078015
• Organism: Enterococcus faecalis strain SY-1

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: KUO14_RS07625
• Protein ID: WP_002401483.1
• Coordinates: 1551585..1552448 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: KUO14_RS07630
• Protein ID: WP_000301765.1
• Coordinates: 1552450..1552722 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUO14_RS07595 (1547383)	1547383..1548150	-	768	WP_002357481.1	ribonuclease HII	-
KUO14_RS07600 (1548152)	1548152..1549003	-	852	WP_002357480.1	ribosome biogenesis GTPase YlqF	-
KUO14_RS07605 (1549279)	1549279..1549710	-	432	Protein_1471	2-dehydropantoate 2-reductase	-
KUO14_RS07610 (1549766)	1549766..1550446	-	681	WP_010710189.1	IS6-like element IS1216 family transposase	-
KUO14_RS07615 (1550520)	1550520..1550807	-	288	Protein_1473	DnaJ domain-containing protein	-
KUO14_RS07620 (1550827)	1550827..1551144	-	318	WP_002333463.1	hypothetical protein	-
KUO14_RS07625 (1551585)	1551585..1552448	-	864	WP_002401483.1	zeta toxin family protein	Toxin
KUO14_RS07630 (1552450)	1552450..1552722	-	273	WP_000301765.1	antitoxin	Antitoxin
KUO14_RS07635 (1552739)	1552739..1552954	-	216	WP_002295735.1	peptide-binding protein	-
KUO14_RS07640 (1553053)	1553053..1553949	-	897	WP_002387620.1	ParA family protein	-
KUO14_RS07645 (1554052)	1554052..1554312	-	261	Protein_1479	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
KUO14_RS07650 (1554482)	1554482..1555219	-	738	WP_002292226.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
KUO14_RS07655 (1555344)	1555344..1555439	-	96	WP_001809736.1	23S rRNA methyltransferase attenuation leader peptide	-
KUO14_RS07660 (1555508)	1555508..1555630	-	123	Protein_1482	peptide-binding protein	-
KUO14_RS07665 (1555750)	1555750..1557276	-	1527	WP_256128437.1	type IA DNA topoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	erm(B) / ant(6)-Ia / aac(6')-aph(2'')	-	1549766..1571174	21408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-213)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32486.06 Da
• Isoelectric Point: 6.9965

>T208890 WP_002401483.1 NZ_CP078015:c1552448-1551585 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T208890 NZ_CP103752:c17581-16256 [Enterobacter asburiae]
ATGAGCCGGAAACAGCAGCGTCTGGCAATCTGGATGAACGGAATCAAAGTCGGATACTGGGAAAAAAGTAAAGGGGTAGA
CAGCTTACAATACCTTCCTGACTGGAGTGCAGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTTCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACAGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGCGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGAACAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAAACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCATTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAAGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAGTTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCACTCTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT208890 WP_000301765.1 NZ_CP078015:c1552722-1552450 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT208890 NZ_CP103752:c17879-17586 [Enterobacter asburiae]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGGGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCTATCCTGGGCGTAAAAATCAGCTTCTCCTCGACAACCGCCTCTTCAGAGGGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGAAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3