Detailed information of TA system TA208883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 6525027..6526567
• Replicon: chromosome
• Accession: NZ_CP078013
• Organism: Pseudomonas pergaminensis strain 1008

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KUA23_RS29870
• Protein ID: WP_099493337.1
• Coordinates: 6525293..6526567 (-)
• Length: 425 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KUA23_RS29865
• Protein ID: WP_078050791.1
• Coordinates: 6525027..6525296 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUA23_RS29845 (KUA23_29675)	6521416..6522963	-	1548	WP_252993246.1	PLP-dependent aminotransferase family protein	-
KUA23_RS29850 (KUA23_29680)	6523051..6523689	+	639	WP_252993247.1	FMN-binding negative transcriptional regulator	-
KUA23_RS29855 (KUA23_29685)	6523686..6524132	+	447	WP_078050789.1	GNAT family N-acetyltransferase	-
KUA23_RS29860 (KUA23_29690)	6524143..6524814	+	672	WP_078050790.1	GNAT family protein	-
KUA23_RS29865 (KUA23_29695)	6525027..6525296	-	270	WP_078050791.1	helix-turn-helix transcriptional regulator	Antitoxin
KUA23_RS29870 (KUA23_29700)	6525293..6526567	-	1275	WP_099493337.1	HipA domain-containing protein	Toxin
KUA23_RS29875 (KUA23_29705)	6526919..6528193	+	1275	WP_252993248.1	autotransporter outer membrane beta-barrel domain-containing protein	-
KUA23_RS29880 (KUA23_29710)	6528283..6530091	-	1809	WP_078050793.1	sodium-extruding oxaloacetate decarboxylase subunit alpha	-
KUA23_RS29885 (KUA23_29715)	6530103..6531518	-	1416	WP_003195732.1	acetyl-CoA carboxylase biotin carboxylase subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-349)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 425 a.a.
• Molecular weight: 47111.41 Da
• Isoelectric Point: 5.5952

>T208883 WP_099493337.1 NZ_CP078013:c6526567-6525293 [Pseudomonas pergaminensis]
MTLEFSEPDKGFASPCRFGYESDYLVDHLDHIDTVFAKAVSVRVPLNWAQEAPRNAPAFLHDIAPAGAAKRFLASRFEML
STDNVSSDLFLLARSTPAPIGHMRIKESLQALNDRPPMGFERQEVIDRDNRFLEYAYEQGAAIGGATGAGGEAPKLLLTQ
SHEGLLYPDAVLGDDQASQHWFIKFARNQGLQIDQDILRSEFHYYKALQSLGIETVAVEGLALEMATKPSLWMHRFDRHV
SDQGVERFAVESIYSLAGVTKPGSAMSHLDVLLQLAGLWQQAGQGEQLTDLVADYLRRDLLNKVLGNSDNHGRNTSIIRG
PDSLRLAPIYDLAPMVMDPEGVTRTTKWPRPLEVAGDIDWRGVCNALAPVINPTDAFERLRTDAQRLRALPDILVDSGLP
KVTLNHPSIALRNLDQRLRSWGLQ

Nucleotide

• Length: 1275 bp

>T208883 NZ_CP103751:1717112-1717633 [Enterobacter asburiae]
GTGGAGAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTATGACTTCGAGGATTTTGATTGTGGCGAACCCTCGCT
AAATGCCTTCCTTGCTGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGCCTACCTGCTTAAAGAGCGCGATC
GTCCGCGTGTCCTGGGTTATTACACGTTATCGGGAAGCTGTTTTGAAAGAGCGATGCTTCCTTCTAAGACTCAGCAGCGC
CGGATCCCTTATATCAACGTACCCAGCGTGACGCTGGGACGTCTGGCTGTAGATAAAACACTACAGGGAAACGAGTGGGG
AACAACGCTCGTTGCGCATGCTATGCGCGTTGTCTACCTGGCTTCACAGGCTGTGGGGGTACACGGAATATTTGTTGATG
CCCTTAACGAGCGGGCAAAACGATTTTATCTGAAGCAGGGCTTTATCCCGCTGACAGGTGAAAACAGCCATTCTTTGTTC
TTCCCTACAAAATCTATCGAACGGCTGTTTGAGCAAGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9877.51 Da
• Isoelectric Point: 10.9066

>AT208883 WP_078050791.1 NZ_CP078013:c6525296-6525027 [Pseudomonas pergaminensis]
MSLSMSQRAQVIESIEQGLSDGSLEIGDAVRRLRTEVTGLHQAQFAKMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGL
KMGVVKVRR

Nucleotide

• Length: 270 bp

>AT208883 NZ_CP103751:1716836-1717105 [Enterobacter asburiae]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATTGAGCAGCACCGTCGTC
TCGTATTAAACGAAGCATCATGGACTGCGGTGATGGATGCCATCGACAATCCGCCTGAGCCAAATGAACGCCTGAAACGA
GCAGCAAAACGGCTTCGAAACATGGAGTGA