Detailed information of TA system TA208881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2020623..2021227
• Replicon: chromosome
• Accession: NZ_CP078013
• Organism: Pseudomonas pergaminensis strain 1008

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1U9PV57
• Locus tag: KUA23_RS09300
• Protein ID: WP_073859991.1
• Coordinates: 2020623..2020811 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KUA23_RS09305
• Protein ID: WP_078047607.1
• Coordinates: 2020823..2021227 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUA23_RS09275 (KUA23_09225)	2016313..2017032	+	720	WP_252994266.1	phosphonate C-P lyase system protein PhnL	-
KUA23_RS09280 (KUA23_09230)	2017022..2018167	+	1146	WP_214498150.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
KUA23_RS09285 (KUA23_09235)	2018167..2018739	+	573	WP_078047604.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
KUA23_RS09290 (KUA23_09240)	2018730..2019479	+	750	WP_252993809.1	phosphonate metabolism protein PhnP	-
KUA23_RS09295 (KUA23_09245)	2019562..2020440	-	879	WP_078047606.1	LysR family transcriptional regulator	-
KUA23_RS09300 (KUA23_09250)	2020623..2020811	+	189	WP_073859991.1	type II toxin-antitoxin system HicA family toxin	Toxin
KUA23_RS09305 (KUA23_09255)	2020823..2021227	+	405	WP_078047607.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KUA23_RS09310 (KUA23_09260)	2021621..2023150	+	1530	WP_252993810.1	M10 family metallopeptidase C-terminal domain-containing protein	-
KUA23_RS09315 (KUA23_09265)	2023393..2025126	+	1734	WP_252993811.1	M10 family metallopeptidase C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7192.39 Da
• Isoelectric Point: 11.2000

>T208881 WP_073859991.1 NZ_CP078013:2020623-2020811 [Pseudomonas pergaminensis]
VQSRLLIKELQEAGWVLDRVTGSHHIFTHRYSPYTLPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T208881 NZ_CP103751:498222-498506 [Enterobacter asburiae]
ATGACTTATGAACTGGCATTTGACCCGAGGGCGTTAAAGGAGTGGTGCAAGCTGGGGCAGACTGTAAAAGACCTGTTTAA
GAAGAAACTTGCGGACGTGCTGGTGAACCCCAGGGTCGAATCGGCCCGCCTGCATGGTCTGCCGGATTGCTACAAAATAA
AACTCAGAACGCAGGGTTATCGTTTGGTCTATCAGGTTCAGGACAACGTTGTAACGGTATTTGTTATCGCTATCGGTAAG
CGAGAGAAATCAGCTGTTTATCATGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14745.73 Da
• Isoelectric Point: 4.4776

>AT208881 WP_078047607.1 NZ_CP078013:2020823-2021227 [Pseudomonas pergaminensis]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGTIPMPTSVAQYHAHEDYAGMGWGM
LELDVTPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT208881 NZ_CP103751:497990-498232 [Enterobacter asburiae]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACGGAAGCCGTGCGATTACTGTTTCAGTACGTCGCGGAGACGGGACGTATGCCGGTGAAAACGGTCACGATTAGCGACA
GCGAAGATGCGCTGATACAGACGGTCCGGGAGCGACTGGCGAATCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A1U9PV57