Detailed information of TA system TA208878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5320181..5320767
• Replicon: chromosome
• Accession: NZ_CP078009
• Organism: Pseudomonas aeruginosa strain FAHZU31

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: KUU74_RS24840
• Protein ID: WP_003120987.1
• Coordinates: 5320468..5320767 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KUU74_RS24835
• Protein ID: WP_003448662.1
• Coordinates: 5320181..5320471 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU74_RS24815 (KUU74_24810)	5315332..5315541	+	210	WP_003105733.1	cold-shock protein	-
KUU74_RS24820 (KUU74_24815)	5315763..5317652	+	1890	WP_016851610.1	hypothetical protein	-
KUU74_RS24825 (KUU74_24820)	5317649..5319625	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
KUU74_RS24830 (KUU74_24825)	5319766..5320110	+	345	WP_016851612.1	hypothetical protein	-
KUU74_RS24835 (KUU74_24830)	5320181..5320471	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
KUU74_RS24840 (KUU74_24835)	5320468..5320767	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU74_RS24845 (KUU74_24840)	5320969..5322093	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
KUU74_RS24850 (KUU74_24845)	5322093..5323802	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
KUU74_RS24855 (KUU74_24850)	5323806..5325131	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
KUU74_RS24860 (KUU74_24855)	5325121..5325654	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5293346..5379572	86226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T208878 WP_003120987.1 NZ_CP078009:c5320767-5320468 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T208878 NZ_CP103746:112186-112602 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT208878 WP_003448662.1 NZ_CP078009:c5320471-5320181 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT208878 NZ_CP103746:111959-112189 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: G8CP73