Detailed information of TA system TA208875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2535820..2536336
• Replicon: chromosome
• Accession: NZ_CP078009
• Organism: Pseudomonas aeruginosa strain FAHZU31

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KUU74_RS12185
• Protein ID: WP_222829503.1
• Coordinates: 2536055..2536336 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A482UCX2
• Locus tag: KUU74_RS12180
• Protein ID: WP_033942931.1
• Coordinates: 2535820..2536065 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU74_RS12160 (KUU74_12160)	2531052..2532668	-	1617	WP_010794484.1	hypothetical protein	-
KUU74_RS12165 (KUU74_12165)	2532679..2533836	-	1158	WP_010794485.1	site-specific integrase	-
KUU74_RS31400 (KUU74_12170)	2533934..2534068	-	135	WP_261990960.1	hypothetical protein	-
KUU74_RS12170 (KUU74_12175)	2534061..2534498	-	438	WP_222829520.1	S24 family peptidase	-
KUU74_RS12175 (KUU74_12180)	2534937..2535635	+	699	WP_222829502.1	inovirus Gp2 family protein	-
KUU74_RS12180 (KUU74_12185)	2535820..2536065	+	246	WP_033942931.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KUU74_RS12185 (KUU74_12190)	2536055..2536336	+	282	WP_222829503.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU74_RS12190 (KUU74_12195)	2536405..2536578	-	174	WP_222829504.1	hypothetical protein	-
KUU74_RS12195 (KUU74_12200)	2536589..2537086	-	498	WP_222829505.1	DNA repair protein RadC	-
KUU74_RS12200 (KUU74_12205)	2537058..2538008	-	951	WP_222829506.1	hydrolase or metal-binding protein	-
KUU74_RS12205 (KUU74_12210)	2538101..2539105	-	1005	WP_222829507.1	YqaJ viral recombinase family protein	-
KUU74_RS12210 (KUU74_12215)	2539183..2540151	-	969	WP_222829508.1	DUF932 domain-containing protein	-
KUU74_RS12215 (KUU74_12220)	2540249..2540578	-	330	WP_261990959.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2501401..2548208	46807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10777.67 Da
• Isoelectric Point: 10.7574

>T208875 WP_222829503.1 NZ_CP078009:2536055-2536336 [Pseudomonas aeruginosa]
MTYKLEFLPSALKEWEKLGHTLREQAKKKLRERLEAPKVQTDALRDLPGHYKIKLRTAGYRLVYRVEDDRVVVVVVAVGK
RERGAAYKAAAKR

Nucleotide

• Length: 282 bp

>T208875 NZ_CP103745:c4410669-4410496 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8653.98 Da
• Isoelectric Point: 4.1469

>AT208875 WP_033942931.1 NZ_CP078009:2535820-2536065 [Pseudomonas aeruginosa]
MQSVLADVAVSVSELKKNPTGVMAGAGGMPVAVLNHNRVMGYMVPAELYEQMLDRLDDLDLIDVVNARSGEKGVPVNLDD
L

Nucleotide

• Length: 246 bp

>AT208875 NZ_CP103745:c4410993-4410697 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A482UCX2