Detailed information of TA system TA208869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3580651..3581872
• Replicon: chromosome
• Accession: NZ_CP078007
• Organism: Pseudomonas aeruginosa strain FAHZU40

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KUU69_RS16595
• Protein ID: WP_003114949.1
• Coordinates: 3581294..3581872 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KUU69_RS16590
• Protein ID: WP_003088112.1
• Coordinates: 3580651..3581205 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU69_RS16565 (KUU69_16555)	3576219..3576770	+	552	WP_003102727.1	XRE family transcriptional regulator	-
KUU69_RS16570 (KUU69_16560)	3576767..3577165	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
KUU69_RS16575 (KUU69_16565)	3577178..3577501	-	324	WP_034038313.1	multidrug efflux SMR transporter	-
KUU69_RS16580 (KUU69_16570)	3577832..3578293	+	462	WP_034038312.1	(2Fe-2S)-binding protein	-
KUU69_RS16585 (KUU69_16575)	3578298..3580493	+	2196	WP_003122807.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
KUU69_RS16590 (KUU69_16580)	3580651..3581205	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
KUU69_RS16595 (KUU69_16585)	3581294..3581872	+	579	WP_003114949.1	HD domain-containing protein	Toxin
KUU69_RS16600 (KUU69_16590)	3581980..3583167	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
KUU69_RS16605 (KUU69_16595)	3583157..3585328	-	2172	WP_042853313.1	type I secretion system permease/ATPase	-
KUU69_RS16610 (KUU69_16600)	3585318..3586595	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21897.75 Da
• Isoelectric Point: 5.3655

>T208869 WP_003114949.1 NZ_CP078007:3581294-3581872 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAVRRDA

Nucleotide

• Length: 579 bp

>T208869 NZ_CP103745:c2890915-2890541 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATAACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT208869 WP_003088112.1 NZ_CP078007:3580651-3581205 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT208869 NZ_CP103745:c2891339-2890962 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCACCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGAAGCTGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCACCCGCAATCACCGTATAA