Detailed information of TA system TA208864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 4674052..4675747
• Replicon: chromosome
• Accession: NZ_CP078006
• Organism: Pseudomonas aeruginosa strain NDTH7329

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: KUU80_RS21935
• Protein ID: WP_003112901.1
• Coordinates: 4674878..4675747 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: KUU80_RS21930
• Protein ID: WP_003112902.1
• Coordinates: 4674052..4674885 (-)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU80_RS21910 (KUU80_21905)	4669283..4670767	-	1485	WP_031756606.1	aldehyde dehydrogenase	-
KUU80_RS21915 (KUU80_21910)	4670779..4671582	-	804	WP_003453564.1	aspartate dehydrogenase	-
KUU80_RS21920 (KUU80_21915)	4671593..4673275	-	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
KUU80_RS21925 (KUU80_21920)	4673268..4674065	-	798	WP_017001845.1	SDR family oxidoreductase	-
KUU80_RS21930 (KUU80_21925)	4674052..4674885	-	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
KUU80_RS21935 (KUU80_21930)	4674878..4675747	-	870	WP_003112901.1	alpha/beta hydrolase	Toxin
KUU80_RS21940 (KUU80_21935)	4675756..4676286	-	531	WP_003112900.1	cupin domain-containing protein	-
KUU80_RS21945 (KUU80_21940)	4676300..4677061	-	762	WP_003112899.1	SDR family oxidoreductase	-
KUU80_RS21950 (KUU80_21945)	4677067..4677843	-	777	WP_003112898.1	ABC transporter permease	-
KUU80_RS21955 (KUU80_21950)	4677840..4678847	-	1008	WP_003162724.1	ABC transporter substrate-binding protein	-
KUU80_RS21960 (KUU80_21955)	4678888..4679739	-	852	WP_003130104.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T208864 WP_003112901.1 NZ_CP078006:c4675747-4674878 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T208864 NZ_CP103745:2066496-2066599 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT208864 WP_003112902.1 NZ_CP078006:c4674885-4674052 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT208864 NZ_CP103745:c2066637-2066366 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1