Detailed information of TA system TA208861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 1502579..1503837
• Replicon: chromosome
• Accession: NZ_CP078006
• Organism: Pseudomonas aeruginosa strain NDTH7329

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: KUU80_RS07190
• Protein ID: WP_003112528.1
• Coordinates: 1503082..1503837 (+)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: KUU80_RS07185
• Protein ID: WP_003086329.1
• Coordinates: 1502579..1502947 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU80_RS07165 (KUU80_07165)	1498742..1499017	+	276	WP_169057979.1	zinc ribbon domain-containing protein	-
KUU80_RS07170 (KUU80_07170)	1499020..1499496	-	477	WP_003112531.1	hypothetical protein	-
KUU80_RS07175 (KUU80_07175)	1499575..1501164	+	1590	WP_074231416.1	aldehyde dehydrogenase family protein	-
KUU80_RS07180 (KUU80_07180)	1501220..1502506	+	1287	WP_003163134.1	FAD-binding oxidoreductase	-
KUU80_RS07185 (KUU80_07185)	1502579..1502947	+	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
KUU80_RS07190 (KUU80_07190)	1503082..1503837	+	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
KUU80_RS07195 (KUU80_07195)	1504605..1505966	+	1362	WP_073647918.1	DNA recombination protein RmuC	-
KUU80_RS07200 (KUU80_07200)	1506121..1508664	-	2544	WP_031755663.1	acyl-homoserine-lactone acylase QuiP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T208861 WP_003112528.1 NZ_CP078006:1503082-1503837 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T208861 NZ_CP103745:1845217-1845468 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT208861 WP_003086329.1 NZ_CP078006:1502579-1502947 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT208861 NZ_CP103745:1845465-1845719 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7