Detailed information of TA system TA208860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1427441..1428093
• Replicon: chromosome
• Accession: NZ_CP078006
• Organism: Pseudomonas aeruginosa strain NDTH7329

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KUU80_RS06775
• Protein ID: WP_003134109.1
• Coordinates: 1427683..1428093 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KUU80_RS06770
• Protein ID: WP_170869361.1
• Coordinates: 1427441..1427683 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU80_RS06755 (KUU80_06755)	1422449..1424338	-	1890	WP_191519263.1	hypothetical protein	-
KUU80_RS06760 (KUU80_06760)	1424450..1425196	-	747	WP_223723588.1	hypothetical protein	-
KUU80_RS06765 (KUU80_06765)	1425210..1427135	-	1926	WP_191519261.1	type I DNA topoisomerase	-
KUU80_RS06770 (KUU80_06770)	1427441..1427683	+	243	WP_170869361.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KUU80_RS06775 (KUU80_06775)	1427683..1428093	+	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
KUU80_RS06780 (KUU80_06780)	1428109..1428270	+	162	WP_191519260.1	hypothetical protein	-
KUU80_RS06785 (KUU80_06785)	1428320..1428538	+	219	WP_023083218.1	hypothetical protein	-
KUU80_RS06790 (KUU80_06790)	1428604..1428801	-	198	WP_191519259.1	CrpP family ICE-associated protein	-
KUU80_RS06795 (KUU80_06795)	1429300..1429788	-	489	WP_023104904.1	single-stranded DNA-binding protein	-
KUU80_RS06800 (KUU80_06800)	1429802..1430332	-	531	WP_121372660.1	DUF3158 family protein	-
KUU80_RS06805 (KUU80_06805)	1430338..1431066	-	729	WP_009315862.1	TIGR03761 family integrating conjugative element protein	-
KUU80_RS33410	1431223..1431735	-	513	WP_223697281.1	hypothetical protein	-
KUU80_RS06820 (KUU80_06820)	1431732..1433057	-	1326	WP_191519258.1	STY4528 family pathogenicity island replication protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA	1319708..1446555	126847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (11-37)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T208860 WP_003134109.1 NZ_CP078006:1427683-1428093 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T208860 NZ_CP103745:1067570-1067905 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8909.94 Da
• Isoelectric Point: 4.4230

>AT208860 WP_170869361.1 NZ_CP078006:1427441-1427683 [Pseudomonas aeruginosa]
MRCVVEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 243 bp

>AT208860 NZ_CP103745:1067322-1067570 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA