Detailed information of TA system TA208857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5650559..5651145
• Replicon: chromosome
• Accession: NZ_CP078004
• Organism: Pseudomonas aeruginosa strain QZPH16

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: KUU76_RS26235
• Protein ID: WP_003120987.1
• Coordinates: 5650846..5651145 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KUU76_RS26230
• Protein ID: WP_003448662.1
• Coordinates: 5650559..5650849 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU76_RS26215 (KUU76_26210)	5646141..5648030	+	1890	WP_196994395.1	hypothetical protein	-
KUU76_RS26220 (KUU76_26215)	5648027..5650003	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
KUU76_RS26225 (KUU76_26220)	5650144..5650488	+	345	WP_016851612.1	hypothetical protein	-
KUU76_RS26230 (KUU76_26225)	5650559..5650849	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
KUU76_RS26235 (KUU76_26230)	5650846..5651145	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU76_RS26240 (KUU76_26235)	5651347..5652471	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
KUU76_RS26245 (KUU76_26240)	5652471..5654180	+	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
KUU76_RS26250 (KUU76_26245)	5654184..5655509	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
KUU76_RS26255 (KUU76_26250)	5655499..5656032	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5622529..5709742	87213

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T208857 WP_003120987.1 NZ_CP078004:c5651145-5650846 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T208857 NZ_CP103745:717023-717319 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT208857 WP_003448662.1 NZ_CP078004:c5650849-5650559 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT208857 NZ_CP103745:717321-717716 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: G8CP73