Detailed information of TA system TA208856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4793885..4794525
• Replicon: chromosome
• Accession: NZ_CP078004
• Organism: Pseudomonas aeruginosa strain QZPH16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KUU76_RS22240
• Protein ID: WP_003134109.1
• Coordinates: 4793885..4794295 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KUU76_RS22245
• Protein ID: WP_031634724.1
• Coordinates: 4794295..4794525 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU76_RS22195 (KUU76_22190)	4788921..4790246	+	1326	WP_191519258.1	STY4528 family pathogenicity island replication protein	-
KUU76_RS32915	4790243..4790755	+	513	WP_223697281.1	hypothetical protein	-
KUU76_RS22210 (KUU76_22205)	4790912..4791640	+	729	WP_009315862.1	TIGR03761 family integrating conjugative element protein	-
KUU76_RS22215 (KUU76_22210)	4791646..4792176	+	531	WP_121372660.1	DUF3158 family protein	-
KUU76_RS22220 (KUU76_22215)	4792190..4792678	+	489	WP_023104904.1	single-stranded DNA-binding protein	-
KUU76_RS22225 (KUU76_22220)	4793177..4793374	+	198	WP_191519259.1	CrpP family ICE-associated protein	-
KUU76_RS22230 (KUU76_22225)	4793440..4793658	-	219	WP_023083218.1	hypothetical protein	-
KUU76_RS22235 (KUU76_22230)	4793708..4793869	-	162	WP_191519260.1	hypothetical protein	-
KUU76_RS22240 (KUU76_22235)	4793885..4794295	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
KUU76_RS22245 (KUU76_22240)	4794295..4794525	-	231	WP_031634724.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KUU76_RS22250 (KUU76_22245)	4794843..4796768	+	1926	WP_191519261.1	type I DNA topoisomerase	-
KUU76_RS22255 (KUU76_22250)	4796782..4797528	+	747	WP_223723588.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA	4775422..4902269	126847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T208856 WP_003134109.1 NZ_CP078004:c4794295-4793885 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T208856 NZ_CP103745:652448-652762 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT208856 WP_031634724.1 NZ_CP078004:c4794525-4794295 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT208856 NZ_CP103745:652759-653175 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA