Detailed information of TA system TA208852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1673736..1675431
• Replicon: chromosome
• Accession: NZ_CP078004
• Organism: Pseudomonas aeruginosa strain QZPH16

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: KUU76_RS07940
• Protein ID: WP_003112901.1
• Coordinates: 1673736..1674605 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: KUU76_RS07945
• Protein ID: WP_003112902.1
• Coordinates: 1674598..1675431 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU76_RS07915 (KUU76_07915)	1669744..1670595	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
KUU76_RS07920 (KUU76_07920)	1670636..1671643	+	1008	WP_003162724.1	ABC transporter substrate-binding protein	-
KUU76_RS07925 (KUU76_07925)	1671640..1672416	+	777	WP_003112898.1	ABC transporter permease	-
KUU76_RS07930 (KUU76_07930)	1672422..1673183	+	762	WP_003112899.1	SDR family oxidoreductase	-
KUU76_RS07935 (KUU76_07935)	1673197..1673727	+	531	WP_003112900.1	cupin domain-containing protein	-
KUU76_RS07940 (KUU76_07940)	1673736..1674605	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
KUU76_RS07945 (KUU76_07945)	1674598..1675431	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
KUU76_RS07950 (KUU76_07950)	1675418..1676215	+	798	WP_017001845.1	SDR family oxidoreductase	-
KUU76_RS07955 (KUU76_07955)	1676208..1677890	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
KUU76_RS07960 (KUU76_07960)	1677901..1678704	+	804	WP_003453564.1	aspartate dehydrogenase	-
KUU76_RS07965 (KUU76_07965)	1678716..1680200	+	1485	WP_031756606.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T208852 WP_003112901.1 NZ_CP078004:1673736-1674605 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T208852 NZ_CP103743:134757-135062 [Escherichia coli O25b:H4-ST131]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT208852 WP_003112902.1 NZ_CP078004:1674598-1675431 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT208852 NZ_CP103743:134537-134755 [Escherichia coli O25b:H4-ST131]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1