Detailed information of TA system TA208849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5446068..5446654
• Replicon: chromosome
• Accession: NZ_CP078002
• Organism: Pseudomonas aeruginosa strain QZPH21

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: KUU68_RS25325
• Protein ID: WP_003120987.1
• Coordinates: 5446355..5446654 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KUU68_RS25320
• Protein ID: WP_003448662.1
• Coordinates: 5446068..5446358 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU68_RS25305 (KUU68_25300)	5441650..5443539	+	1890	WP_196994395.1	hypothetical protein	-
KUU68_RS25310 (KUU68_25305)	5443536..5445512	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
KUU68_RS25315 (KUU68_25310)	5445653..5445997	+	345	WP_016851612.1	hypothetical protein	-
KUU68_RS25320 (KUU68_25315)	5446068..5446358	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
KUU68_RS25325 (KUU68_25320)	5446355..5446654	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU68_RS25330 (KUU68_25325)	5446856..5447980	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
KUU68_RS25335 (KUU68_25330)	5447980..5449689	+	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
KUU68_RS25340 (KUU68_25335)	5449693..5451018	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
KUU68_RS25345 (KUU68_25340)	5451008..5451541	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5418038..5505251	87213

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T208849 WP_003120987.1 NZ_CP078002:c5446654-5446355 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T208849 NZ_CP103742:c4350371-4350198 [Escherichia coli O25b:H4-ST131]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT208849 WP_003448662.1 NZ_CP078002:c5446358-5446068 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT208849 NZ_CP103742:c4350695-4350399 [Escherichia coli O25b:H4-ST131]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: G8CP73