Detailed information of TA system TA208848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4589394..4590034
• Replicon: chromosome
• Accession: NZ_CP078002
• Organism: Pseudomonas aeruginosa strain QZPH21

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KUU68_RS21330
• Protein ID: WP_003134109.1
• Coordinates: 4589394..4589804 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KUU68_RS21335
• Protein ID: WP_031634724.1
• Coordinates: 4589804..4590034 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU68_RS21285 (KUU68_21280)	4584430..4585755	+	1326	WP_191519258.1	STY4528 family pathogenicity island replication protein	-
KUU68_RS31945	4585752..4586264	+	513	WP_223697281.1	hypothetical protein	-
KUU68_RS21300 (KUU68_21295)	4586421..4587149	+	729	WP_009315862.1	TIGR03761 family integrating conjugative element protein	-
KUU68_RS21305 (KUU68_21300)	4587155..4587685	+	531	WP_121372660.1	DUF3158 family protein	-
KUU68_RS21310 (KUU68_21305)	4587699..4588187	+	489	WP_023104904.1	single-stranded DNA-binding protein	-
KUU68_RS21315 (KUU68_21310)	4588686..4588883	+	198	WP_191519259.1	CrpP family ICE-associated protein	-
KUU68_RS21320 (KUU68_21315)	4588949..4589167	-	219	WP_023083218.1	hypothetical protein	-
KUU68_RS21325 (KUU68_21320)	4589217..4589378	-	162	WP_191519260.1	hypothetical protein	-
KUU68_RS21330 (KUU68_21325)	4589394..4589804	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
KUU68_RS21335 (KUU68_21330)	4589804..4590034	-	231	WP_031634724.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KUU68_RS21340 (KUU68_21335)	4590352..4592277	+	1926	WP_191519261.1	type I DNA topoisomerase	-
KUU68_RS21345 (KUU68_21340)	4592291..4593037	+	747	WP_223723588.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA	4570931..4697778	126847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T208848 WP_003134109.1 NZ_CP078002:c4589804-4589394 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T208848 NZ_CP103742:c4142201-4141824 [Escherichia coli O25b:H4-ST131]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT208848 WP_031634724.1 NZ_CP078002:c4590034-4589804 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT208848 NZ_CP103742:c4142658-4142290 [Escherichia coli O25b:H4-ST131]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA