Detailed information of TA system TA208847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4513649..4514907
• Replicon: chromosome
• Accession: NZ_CP078002
• Organism: Pseudomonas aeruginosa strain QZPH21

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: KUU68_RS20920
• Protein ID: WP_003112528.1
• Coordinates: 4513649..4514404 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: KUU68_RS20925
• Protein ID: WP_003086329.1
• Coordinates: 4514539..4514907 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU68_RS20910 (KUU68_20905)	4508822..4511365	+	2544	WP_031755663.1	acyl-homoserine-lactone acylase QuiP	-
KUU68_RS20915 (KUU68_20910)	4511520..4512881	-	1362	WP_073647918.1	DNA recombination protein RmuC	-
KUU68_RS20920 (KUU68_20915)	4513649..4514404	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
KUU68_RS20925 (KUU68_20920)	4514539..4514907	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
KUU68_RS20930 (KUU68_20925)	4514980..4516266	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
KUU68_RS20935 (KUU68_20930)	4516322..4517911	-	1590	WP_074231416.1	aldehyde dehydrogenase family protein	-
KUU68_RS20940 (KUU68_20935)	4517990..4518466	+	477	WP_003112531.1	hypothetical protein	-
KUU68_RS20945 (KUU68_20940)	4518469..4518744	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T208847 WP_003112528.1 NZ_CP078002:c4514404-4513649 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T208847 NZ_CP103742:c4071544-4071257 [Escherichia coli O25b:H4-ST131]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT208847 WP_003086329.1 NZ_CP078002:c4514907-4514539 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT208847 NZ_CP103742:c4071270-4070968 [Escherichia coli O25b:H4-ST131]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7