Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RelE-RHH |
| Location | 5391..5941 | Replicon | plasmid pSRRSH1120-KPC |
| Accession | NZ_CP078000 | ||
| Organism | Pseudomonas aeruginosa strain SRRSH1120 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | - |
| Locus tag | KUU71_RS32545 | Protein ID | WP_019727125.1 |
| Coordinates | 5391..5666 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | A0A6B1YL09 |
| Locus tag | KUU71_RS32550 | Protein ID | WP_019727126.1 |
| Coordinates | 5666..5941 (-) | Length | 92 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KUU71_RS32510 (KUU71_32505) | 466..921 | + | 456 | WP_124175138.1 | hypothetical protein | - |
| KUU71_RS32515 (KUU71_32510) | 2001..2678 | + | 678 | WP_034018017.1 | SOS response-associated peptidase | - |
| KUU71_RS32520 (KUU71_32515) | 2787..3089 | + | 303 | WP_023131919.1 | anti-CRISPR protein AcrE1 | - |
| KUU71_RS32525 (KUU71_32520) | 3112..3534 | + | 423 | WP_196994196.1 | anti-CRISPR protein AcrF3 | - |
| KUU71_RS32530 (KUU71_32525) | 3547..3786 | + | 240 | WP_196994197.1 | anti-CRISPR protein | - |
| KUU71_RS33450 | 3783..3986 | + | 204 | WP_031757130.1 | helix-turn-helix transcriptional regulator | - |
| KUU71_RS32535 (KUU71_32530) | 4082..4402 | + | 321 | WP_196994198.1 | hypothetical protein | - |
| KUU71_RS32540 (KUU71_32535) | 4884..5300 | + | 417 | WP_196994199.1 | hypothetical protein | - |
| KUU71_RS32545 (KUU71_32540) | 5391..5666 | - | 276 | WP_019727125.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| KUU71_RS32550 (KUU71_32545) | 5666..5941 | - | 276 | WP_019727126.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| KUU71_RS32555 (KUU71_32550) | 6216..6602 | + | 387 | WP_019727127.1 | hypothetical protein | - |
| KUU71_RS32560 (KUU71_32555) | 6663..6956 | + | 294 | WP_019727128.1 | hypothetical protein | - |
| KUU71_RS32565 (KUU71_32560) | 7280..7588 | + | 309 | WP_019727129.1 | TrfB-related DNA-binding protein | - |
| KUU71_RS32570 (KUU71_32565) | 8238..8690 | + | 453 | WP_019727132.1 | DNA repair protein RadC | - |
| KUU71_RS32575 (KUU71_32570) | 9128..9376 | + | 249 | WP_058130600.1 | hypothetical protein | - |
| KUU71_RS32580 (KUU71_32575) | 9426..9677 | - | 252 | WP_058130599.1 | hypothetical protein | - |
| KUU71_RS32585 (KUU71_32580) | 9736..10020 | + | 285 | WP_019727136.1 | hypothetical protein | - |
| KUU71_RS32590 (KUU71_32585) | 10063..10440 | + | 378 | WP_019727137.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaKPC-2 | - | 1..106679 | 106679 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10779.36 Da Isoelectric Point: 7.1659
>T208842 WP_019727125.1 NZ_CP078000:c5666-5391 [Pseudomonas aeruginosa]
MELKWTSKGLSDVARLYEFLATVNREAAARTVQQLTSAPTALLTNPRLGERLEEFEPRDVRRILIGHYEMRYEILDSCIY
VLRLWHTREDR
MELKWTSKGLSDVARLYEFLATVNREAAARTVQQLTSAPTALLTNPRLGERLEEFEPRDVRRILIGHYEMRYEILDSCIY
VLRLWHTREDR
Download Length: 276 bp
>T208842 NZ_CP103742:2843606-2843884 [Escherichia coli O25b:H4-ST131]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA
Antitoxin
Download Length: 92 a.a. Molecular weight: 10142.32 Da Isoelectric Point: 4.8433
>AT208842 WP_019727126.1 NZ_CP078000:c5941-5666 [Pseudomonas aeruginosa]
MSTAAKPTRTVTAHVPVDLAERVDAIAERLERSKNWIVRQALSAWIDQEDERSRLTREALDDVDAGRVVDHQAVQAWAES
LGTDSPLPVPR
MSTAAKPTRTVTAHVPVDLAERVDAIAERLERSKNWIVRQALSAWIDQEDERSRLTREALDDVDAGRVVDHQAVQAWAES
LGTDSPLPVPR
Download Length: 276 bp
>AT208842 NZ_CP103742:2843413-2843604 [Escherichia coli O25b:H4-ST131]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGTAAGGAACGGAGCAAGCAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|