Detailed information of TA system TA208839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4691293..4692551
• Replicon: chromosome
• Accession: NZ_CP077999
• Organism: Pseudomonas aeruginosa strain SRRSH1120

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: KUU71_RS21570
• Protein ID: WP_003112528.1
• Coordinates: 4691293..4692048 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: KUU71_RS21575
• Protein ID: WP_003086329.1
• Coordinates: 4692183..4692551 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU71_RS21560 (KUU71_21555)	4686466..4689009	+	2544	WP_031755663.1	acyl-homoserine-lactone acylase QuiP	-
KUU71_RS21565 (KUU71_21560)	4689164..4690525	-	1362	WP_073647918.1	DNA recombination protein RmuC	-
KUU71_RS21570 (KUU71_21565)	4691293..4692048	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
KUU71_RS21575 (KUU71_21570)	4692183..4692551	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
KUU71_RS21580 (KUU71_21575)	4692624..4693910	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
KUU71_RS21585 (KUU71_21580)	4693966..4695555	-	1590	WP_074231416.1	aldehyde dehydrogenase family protein	-
KUU71_RS21590 (KUU71_21585)	4695634..4696110	+	477	WP_003112531.1	hypothetical protein	-
KUU71_RS21595 (KUU71_21590)	4696113..4696388	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T208839 WP_003112528.1 NZ_CP077999:c4692048-4691293 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T208839 NZ_CP103742:2131732-2131834 [Escherichia coli O25b:H4-ST131]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT208839 WP_003086329.1 NZ_CP077999:c4692551-4692183 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT208839 NZ_CP103742:c2131872-2131604 [Escherichia coli O25b:H4-ST131]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7