Detailed information of TA system TA208829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1583118..1584813
• Replicon: chromosome
• Accession: NZ_CP077997
• Organism: Pseudomonas aeruginosa strain SRRSH1521

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: KUU75_RS07370
• Protein ID: WP_003112901.1
• Coordinates: 1583118..1583987 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: KUU75_RS07375
• Protein ID: WP_003112902.1
• Coordinates: 1583980..1584813 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU75_RS07345 (KUU75_07345)	1579126..1579977	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
KUU75_RS07350 (KUU75_07350)	1580018..1581025	+	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
KUU75_RS07355 (KUU75_07355)	1581022..1581798	+	777	WP_003112898.1	ABC transporter permease	-
KUU75_RS07360 (KUU75_07360)	1581804..1582565	+	762	WP_003112899.1	SDR family oxidoreductase	-
KUU75_RS07365 (KUU75_07365)	1582579..1583109	+	531	WP_003112900.1	cupin domain-containing protein	-
KUU75_RS07370 (KUU75_07370)	1583118..1583987	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
KUU75_RS07375 (KUU75_07375)	1583980..1584813	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
KUU75_RS07380 (KUU75_07380)	1584800..1585597	+	798	WP_003112903.1	SDR family oxidoreductase	-
KUU75_RS07385 (KUU75_07385)	1585590..1587272	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
KUU75_RS07390 (KUU75_07390)	1587283..1588086	+	804	WP_003130116.1	aspartate dehydrogenase	-
KUU75_RS07395 (KUU75_07395)	1588098..1589582	+	1485	WP_003453562.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T208829 WP_003112901.1 NZ_CP077997:1583118-1583987 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T208829 NZ_CP103742:188058-188165 [Escherichia coli O25b:H4-ST131]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT208829 WP_003112902.1 NZ_CP077997:1583980-1584813 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT208829 NZ_CP103742:c188009-187944 [Escherichia coli O25b:H4-ST131]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1