Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RelE-RHH |
| Location | 9459..10009 | Replicon | plasmid p2 |
| Accession | NZ_CP077996 | ||
| Organism | Pseudomonas aeruginosa strain SRRSH2790 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | - |
| Locus tag | KUU72_RS32830 | Protein ID | WP_019727125.1 |
| Coordinates | 9459..9734 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | A0A6B1YL09 |
| Locus tag | KUU72_RS32835 | Protein ID | WP_019727126.1 |
| Coordinates | 9734..10009 (-) | Length | 92 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KUU72_RS32785 (KUU72_32780) | 4584..4883 | + | 300 | WP_196994194.1 | hypothetical protein | - |
| KUU72_RS32790 (KUU72_32785) | 4996..5202 | + | 207 | WP_152996423.1 | hypothetical protein | - |
| KUU72_RS32795 (KUU72_32790) | 5229..5483 | + | 255 | WP_196994195.1 | hypothetical protein | - |
| KUU72_RS32800 (KUU72_32795) | 6069..6746 | + | 678 | WP_034018017.1 | SOS response-associated peptidase | - |
| KUU72_RS32805 (KUU72_32800) | 6855..7157 | + | 303 | WP_023131919.1 | anti-CRISPR protein AcrE1 | - |
| KUU72_RS32810 (KUU72_32805) | 7180..7602 | + | 423 | WP_196994196.1 | anti-CRISPR protein AcrF3 | - |
| KUU72_RS32815 (KUU72_32810) | 7615..7854 | + | 240 | WP_196994197.1 | anti-CRISPR protein | - |
| KUU72_RS33345 | 7851..8054 | + | 204 | WP_031757130.1 | helix-turn-helix transcriptional regulator | - |
| KUU72_RS32820 (KUU72_32815) | 8150..8470 | + | 321 | WP_196994198.1 | hypothetical protein | - |
| KUU72_RS32825 (KUU72_32820) | 8952..9368 | + | 417 | WP_196994199.1 | hypothetical protein | - |
| KUU72_RS32830 (KUU72_32825) | 9459..9734 | - | 276 | WP_019727125.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| KUU72_RS32835 (KUU72_32830) | 9734..10009 | - | 276 | WP_019727126.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| KUU72_RS32840 (KUU72_32835) | 10284..10670 | + | 387 | WP_019727127.1 | hypothetical protein | - |
| KUU72_RS32845 (KUU72_32840) | 10731..11024 | + | 294 | WP_019727128.1 | hypothetical protein | - |
| KUU72_RS32850 (KUU72_32845) | 11348..11656 | + | 309 | WP_019727129.1 | TrfB-related DNA-binding protein | - |
| KUU72_RS32855 (KUU72_32850) | 12306..12758 | + | 453 | WP_019727132.1 | DNA repair protein RadC | - |
| KUU72_RS32860 (KUU72_32855) | 13196..13444 | + | 249 | WP_058130600.1 | hypothetical protein | - |
| KUU72_RS32865 (KUU72_32860) | 13494..13745 | - | 252 | WP_058130599.1 | hypothetical protein | - |
| KUU72_RS32870 (KUU72_32865) | 13804..14088 | + | 285 | WP_019727136.1 | hypothetical protein | - |
| KUU72_RS32875 (KUU72_32870) | 14215..14493 | - | 279 | WP_222825899.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..52983 | 52983 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10779.36 Da Isoelectric Point: 7.1659
>T208827 WP_019727125.1 NZ_CP077996:c9734-9459 [Pseudomonas aeruginosa]
MELKWTSKGLSDVARLYEFLATVNREAAARTVQQLTSAPTALLTNPRLGERLEEFEPRDVRRILIGHYEMRYEILDSCIY
VLRLWHTREDR
MELKWTSKGLSDVARLYEFLATVNREAAARTVQQLTSAPTALLTNPRLGERLEEFEPRDVRRILIGHYEMRYEILDSCIY
VLRLWHTREDR
Download Length: 276 bp
>T208827 NZ_CP103740:206206-206511 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
Antitoxin
Download Length: 92 a.a. Molecular weight: 10142.32 Da Isoelectric Point: 4.8433
>AT208827 WP_019727126.1 NZ_CP077996:c10009-9734 [Pseudomonas aeruginosa]
MSTAAKPTRTVTAHVPVDLAERVDAIAERLERSKNWIVRQALSAWIDQEDERSRLTREALDDVDAGRVVDHQAVQAWAES
LGTDSPLPVPR
MSTAAKPTRTVTAHVPVDLAERVDAIAERLERSKNWIVRQALSAWIDQEDERSRLTREALDDVDAGRVVDHQAVQAWAES
LGTDSPLPVPR
Download Length: 276 bp
>AT208827 NZ_CP103740:205986-206204 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|