Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 5649430..5650016 Replicon chromosome
Accession NZ_CP077994
Organism Pseudomonas aeruginosa strain SRRSH2790

Toxin (Protein)


Gene name PumA Uniprot ID G8CP73
Locus tag KUU72_RS26240 Protein ID WP_003120987.1
Coordinates 5649717..5650016 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID -
Locus tag KUU72_RS26235 Protein ID WP_003448662.1
Coordinates 5649430..5649720 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUU72_RS26220 (KUU72_26215) 5645012..5646901 + 1890 WP_196994395.1 hypothetical protein -
KUU72_RS26225 (KUU72_26220) 5646898..5648874 + 1977 WP_016851611.1 DEAD/DEAH box helicase -
KUU72_RS26230 (KUU72_26225) 5649015..5649359 + 345 WP_016851612.1 hypothetical protein -
KUU72_RS26235 (KUU72_26230) 5649430..5649720 - 291 WP_003448662.1 putative addiction module antidote protein Antitoxin
KUU72_RS26240 (KUU72_26235) 5649717..5650016 - 300 WP_003120987.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KUU72_RS26245 (KUU72_26240) 5650218..5651342 + 1125 WP_012076859.1 TcpQ domain-containing protein -
KUU72_RS26250 (KUU72_26245) 5651342..5653051 + 1710 WP_003099757.1 PilN family type IVB pilus formation outer membrane protein -
KUU72_RS26255 (KUU72_26250) 5653055..5654380 + 1326 WP_003099758.1 type 4b pilus protein PilO2 -
KUU72_RS26260 (KUU72_26255) 5654370..5654903 + 534 WP_003099760.1 type IV pilus biogenesis protein PilP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 5621400..5708613 87213


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(47-90)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11133.88 Da        Isoelectric Point: 10.4495

>T208825 WP_003120987.1 NZ_CP077994:c5650016-5649717 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Download         Length: 300 bp

>T208825 NZ_CP103739:c4733048-4732941 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10394.91 Da        Isoelectric Point: 4.7527

>AT208825 WP_003448662.1 NZ_CP077994:c5649720-5649430 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Download         Length: 291 bp

>AT208825 NZ_CP103739:4733096-4733162 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G8CP73

Antitoxin

Source ID Structure

References