Detailed information of TA system TA208824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4792756..4793396
• Replicon: chromosome
• Accession: NZ_CP077994
• Organism: Pseudomonas aeruginosa strain SRRSH2790

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KUU72_RS22245
• Protein ID: WP_003134109.1
• Coordinates: 4792756..4793166 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KUU72_RS22250
• Protein ID: WP_031634724.1
• Coordinates: 4793166..4793396 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU72_RS22200 (KUU72_22195)	4787792..4789117	+	1326	WP_191519258.1	STY4528 family pathogenicity island replication protein	-
KUU72_RS33260	4789114..4789626	+	513	WP_223697281.1	hypothetical protein	-
KUU72_RS22215 (KUU72_22210)	4789783..4790511	+	729	WP_009315862.1	TIGR03761 family integrating conjugative element protein	-
KUU72_RS22220 (KUU72_22215)	4790517..4791047	+	531	WP_121372660.1	DUF3158 family protein	-
KUU72_RS22225 (KUU72_22220)	4791061..4791549	+	489	WP_023104904.1	single-stranded DNA-binding protein	-
KUU72_RS22230 (KUU72_22225)	4792048..4792245	+	198	WP_191519259.1	CrpP family ICE-associated protein	-
KUU72_RS22235 (KUU72_22230)	4792311..4792529	-	219	WP_023083218.1	hypothetical protein	-
KUU72_RS22240 (KUU72_22235)	4792579..4792740	-	162	WP_191519260.1	hypothetical protein	-
KUU72_RS22245 (KUU72_22240)	4792756..4793166	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
KUU72_RS22250 (KUU72_22245)	4793166..4793396	-	231	WP_031634724.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KUU72_RS22255 (KUU72_22250)	4793714..4795639	+	1926	WP_191519261.1	type I DNA topoisomerase	-
KUU72_RS22260 (KUU72_22255)	4795653..4796399	+	747	WP_223723588.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	katA	4774293..4901140	126847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T208824 WP_003134109.1 NZ_CP077994:c4793166-4792756 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T208824 NZ_CP103739:c4317394-4316930 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT208824 WP_031634724.1 NZ_CP077994:c4793396-4793166 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT208824 NZ_CP103739:c4317729-4317394 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA