Detailed information of TA system TA208823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4717011..4718269
• Replicon: chromosome
• Accession: NZ_CP077994
• Organism: Pseudomonas aeruginosa strain SRRSH2790

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: KUU72_RS21835
• Protein ID: WP_003112528.1
• Coordinates: 4717011..4717766 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: KUU72_RS21840
• Protein ID: WP_003086329.1
• Coordinates: 4717901..4718269 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU72_RS21825 (KUU72_21820)	4712184..4714727	+	2544	WP_031755663.1	acyl-homoserine-lactone acylase QuiP	-
KUU72_RS21830 (KUU72_21825)	4714882..4716243	-	1362	WP_073647918.1	DNA recombination protein RmuC	-
KUU72_RS21835 (KUU72_21830)	4717011..4717766	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
KUU72_RS21840 (KUU72_21835)	4717901..4718269	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
KUU72_RS21845 (KUU72_21840)	4718342..4719628	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
KUU72_RS21850 (KUU72_21845)	4719684..4721273	-	1590	WP_074231416.1	aldehyde dehydrogenase family protein	-
KUU72_RS21855 (KUU72_21850)	4721352..4721828	+	477	WP_003112531.1	hypothetical protein	-
KUU72_RS21860 (KUU72_21855)	4721831..4722106	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T208823 WP_003112528.1 NZ_CP077994:c4717766-4717011 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T208823 NZ_CP103739:c4281775-4281461 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT208823 WP_003086329.1 NZ_CP077994:c4718269-4717901 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT208823 NZ_CP103739:c4281464-4281048 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7