Detailed information of TA system TA208822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3752707..3753927
• Replicon: chromosome
• Accession: NZ_CP077994
• Organism: Pseudomonas aeruginosa strain SRRSH2790

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KUU72_RS17220
• Protein ID: WP_003160088.1
• Coordinates: 3753349..3753927 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KUU72_RS17215
• Protein ID: WP_003088112.1
• Coordinates: 3752707..3753261 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU72_RS17190 (KUU72_17190)	3748275..3748826	+	552	WP_003132721.1	XRE family transcriptional regulator	-
KUU72_RS17195 (KUU72_17195)	3748823..3749221	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
KUU72_RS17200 (KUU72_17200)	3749234..3749557	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
KUU72_RS17205 (KUU72_17205)	3749888..3750349	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
KUU72_RS17210 (KUU72_17210)	3750354..3752549	+	2196	WP_003122807.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
KUU72_RS17215 (KUU72_17215)	3752707..3753261	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
KUU72_RS17220 (KUU72_17220)	3753349..3753927	+	579	WP_003160088.1	HD domain-containing protein	Toxin
KUU72_RS17225 (KUU72_17225)	3754036..3755223	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
KUU72_RS17230 (KUU72_17230)	3755213..3757384	-	2172	WP_009876279.1	type I secretion system permease/ATPase	-
KUU72_RS17235 (KUU72_17235)	3757374..3758651	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21816.65 Da
• Isoelectric Point: 5.1625

>T208822 WP_003160088.1 NZ_CP077994:3753349-3753927 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQCLAARRDA

Nucleotide

• Length: 579 bp

>T208822 NZ_CP103739:c4216070-4215774 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT208822 WP_003088112.1 NZ_CP077994:3752707-3753261 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT208822 NZ_CP103739:c4215772-4215377 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA