Detailed information of TA system TA208808

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 5051585..5052193
• Replicon: chromosome
• Accession: NZ_CP077985
• Organism: Pseudomonas aeruginosa strain ZPPH2

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: KUU77_RS23830
• Protein ID: WP_016263850.1
• Coordinates: 5051585..5051932 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: KUU77_RS23835
• Protein ID: WP_003114155.1
• Coordinates: 5051942..5052193 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU77_RS23815 (KUU77_23800)	5048332..5049006	-	675	WP_222821599.1	DUF4194 domain-containing protein	-
KUU77_RS23820 (KUU77_23805)	5049038..5050513	-	1476	WP_222821600.1	DUF3375 domain-containing protein	-
KUU77_RS23825 (KUU77_23810)	5050610..5051221	-	612	WP_222821601.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
KUU77_RS23830 (KUU77_23815)	5051585..5051932	-	348	WP_016263850.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KUU77_RS23835 (KUU77_23820)	5051942..5052193	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KUU77_RS23840 (KUU77_23825)	5052407..5053390	-	984	WP_019725833.1	tyrosine-type recombinase/integrase	-
KUU77_RS23845 (KUU77_23830)	5053390..5054682	-	1293	WP_003123045.1	hypothetical protein	-
KUU77_RS23850 (KUU77_23835)	5054941..5056203	-	1263	WP_033953311.1	zonular occludens toxin domain-containing protein	-
KUU77_RS23855 (KUU77_23840)	5056205..5056555	-	351	WP_003133726.1	DUF2523 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13046.85 Da
• Isoelectric Point: 4.4212

>T208808 WP_016263850.1 NZ_CP077985:c5051932-5051585 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLVPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T208808 NZ_CP103739:c1123191-1122913 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT208808 WP_003114155.1 NZ_CP077985:c5052193-5051942 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT208808 NZ_CP103739:c1123454-1123194 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GTTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355