Detailed information of TA system TA208807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4579190..4580448
• Replicon: chromosome
• Accession: NZ_CP077985
• Organism: Pseudomonas aeruginosa strain ZPPH2

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: KUU77_RS21470
• Protein ID: WP_003112528.1
• Coordinates: 4579190..4579945 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: KUU77_RS21475
• Protein ID: WP_003086329.1
• Coordinates: 4580080..4580448 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KUU77_RS21460 (KUU77_21445)	4574362..4576905	+	2544	WP_108186885.1	acyl-homoserine-lactone acylase QuiP	-
KUU77_RS21465 (KUU77_21450)	4577060..4578421	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
KUU77_RS21470 (KUU77_21455)	4579190..4579945	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
KUU77_RS21475 (KUU77_21460)	4580080..4580448	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
KUU77_RS21480 (KUU77_21465)	4580521..4581807	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
KUU77_RS21485 (KUU77_21470)	4581863..4583452	-	1590	WP_023464367.1	aldehyde dehydrogenase family protein	-
KUU77_RS21490 (KUU77_21475)	4583531..4584007	+	477	WP_004353334.1	elongation factor GreAB	-
KUU77_RS21495 (KUU77_21480)	4584010..4584285	-	276	WP_023088486.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T208807 WP_003112528.1 NZ_CP077985:c4579945-4579190 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T208807 NZ_CP103739:821068-821355 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT208807 WP_003086329.1 NZ_CP077985:c4580448-4580080 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT208807 NZ_CP103739:821342-821644 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7