Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RelE-RHH |
| Location | 10085..10635 | Replicon | plasmid p2 |
| Accession | NZ_CP077979 | ||
| Organism | Pseudomonas aeruginosa strain ZPPH29 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | - |
| Locus tag | KUU70_RS33295 | Protein ID | WP_019727125.1 |
| Coordinates | 10085..10360 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | A0A6B1YL09 |
| Locus tag | KUU70_RS33300 | Protein ID | WP_019727126.1 |
| Coordinates | 10360..10635 (-) | Length | 92 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KUU70_RS33260 (KUU70_33260) | 5851..6153 | + | 303 | WP_023131919.1 | anti-CRISPR protein AcrE1 | - |
| KUU70_RS33265 (KUU70_33265) | 6176..6589 | + | 414 | WP_222829184.1 | anti-CRISPR protein AcrF3 | - |
| KUU70_RS33270 (KUU70_33270) | 6602..6841 | + | 240 | WP_034027474.1 | anti-CRISPR protein | - |
| KUU70_RS33735 | 6838..7041 | + | 204 | WP_031757130.1 | helix-turn-helix transcriptional regulator | - |
| KUU70_RS33275 (KUU70_33275) | 7137..7433 | + | 297 | WP_031757131.1 | hypothetical protein | - |
| KUU70_RS33280 (KUU70_33280) | 7886..8365 | + | 480 | WP_222829185.1 | hypothetical protein | - |
| KUU70_RS33285 (KUU70_33285) | 8422..9597 | + | 1176 | WP_222829186.1 | hypothetical protein | - |
| KUU70_RS33290 (KUU70_33290) | 9578..9994 | + | 417 | WP_033992331.1 | hypothetical protein | - |
| KUU70_RS33295 (KUU70_33295) | 10085..10360 | - | 276 | WP_019727125.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| KUU70_RS33300 (KUU70_33300) | 10360..10635 | - | 276 | WP_019727126.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| KUU70_RS33305 (KUU70_33305) | 10910..11296 | + | 387 | WP_023123731.1 | hypothetical protein | - |
| KUU70_RS33310 (KUU70_33310) | 11357..11650 | + | 294 | WP_019727128.1 | hypothetical protein | - |
| KUU70_RS33315 (KUU70_33315) | 11974..12282 | + | 309 | WP_034055800.1 | TrfB-related DNA-binding protein | - |
| KUU70_RS33320 (KUU70_33320) | 12275..12607 | + | 333 | WP_034055802.1 | hypothetical protein | - |
| KUU70_RS33325 (KUU70_33325) | 12901..13149 | + | 249 | WP_034055805.1 | hypothetical protein | - |
| KUU70_RS33330 (KUU70_33330) | 13199..13450 | - | 252 | WP_019727135.1 | hypothetical protein | - |
| KUU70_RS33335 (KUU70_33335) | 13509..13793 | + | 285 | WP_031757108.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..49798 | 49798 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10779.36 Da Isoelectric Point: 7.1659
>T208796 WP_019727125.1 NZ_CP077979:c10360-10085 [Pseudomonas aeruginosa]
MELKWTSKGLSDVARLYEFLATVNREAAARTVQQLTSAPTALLTNPRLGERLEEFEPRDVRRILIGHYEMRYEILDSCIY
VLRLWHTREDR
MELKWTSKGLSDVARLYEFLATVNREAAARTVQQLTSAPTALLTNPRLGERLEEFEPRDVRRILIGHYEMRYEILDSCIY
VLRLWHTREDR
Download Length: 276 bp
>T208796 NZ_CP103731:c4785345-4784812 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
Antitoxin
Download Length: 92 a.a. Molecular weight: 10142.32 Da Isoelectric Point: 4.8433
>AT208796 WP_019727126.1 NZ_CP077979:c10635-10360 [Pseudomonas aeruginosa]
MSTAAKPTRTVTAHVPVDLAERVDAIAERLERSKNWIVRQALSAWIDQEDERSRLTREALDDVDAGRVVDHQAVQAWAES
LGTDSPLPVPR
MSTAAKPTRTVTAHVPVDLAERVDAIAERLERSKNWIVRQALSAWIDQEDERSRLTREALDDVDAGRVVDHQAVQAWAES
LGTDSPLPVPR
Download Length: 276 bp
>AT208796 NZ_CP103731:c4785622-4785356 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|