Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) slvAT/RES(toxin)
Location 153732..154648 Replicon plasmid pZPPH29-KPC
Accession NZ_CP077978
Organism Pseudomonas aeruginosa strain ZPPH29

Toxin (Protein)


Gene name slvT Uniprot ID A0A212BIN6
Locus tag KUU70_RS31640 Protein ID WP_021018402.1
Coordinates 153732..154256 (-) Length 175 a.a.

Antitoxin (Protein)


Gene name slvA Uniprot ID A0A2I0C738
Locus tag KUU70_RS31645 Protein ID WP_010792483.1
Coordinates 154253..154648 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KUU70_RS31620 (KUU70_31615) 149048..150193 + 1146 WP_021018404.1 glycerate kinase -
KUU70_RS31625 (KUU70_31620) 150615..150806 - 192 WP_010792486.1 hypothetical protein -
KUU70_RS31630 (KUU70_31625) 151184..152239 + 1056 WP_021018403.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
KUU70_RS31635 (KUU70_31630) 152436..153686 + 1251 WP_010792484.1 TerD family protein -
KUU70_RS31640 (KUU70_31635) 153732..154256 - 525 WP_021018402.1 RES family NAD+ phosphorylase Toxin
KUU70_RS31645 (KUU70_31640) 154253..154648 - 396 WP_010792483.1 hypothetical protein Antitoxin
KUU70_RS31650 (KUU70_31645) 154858..156792 - 1935 WP_021018401.1 hypothetical protein -
KUU70_RS31655 (KUU70_31650) 158101..158625 + 525 WP_010792479.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..397554 397554


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(10-167)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19011.47 Da        Isoelectric Point: 4.4540

>T208795 WP_021018402.1 NZ_CP077978:c154256-153732 [Pseudomonas aeruginosa]
MKIIGVQDLPCYRMHTPKWSVAPTSGAGAATQGGRVNRKGLEALYLSLESATAIAEFQQGSAFLPPGLLVSYSLSLDRVI
DFTGGYDDTWAPIWQDFFCDWRKIRFNDGVEPPSWIVGDLAIEADCAGILFESVANPGGRNLVLFTDQLPVHGNIVVNDP
RGDLPTDQSSWTRP

Download         Length: 525 bp

>T208795 NZ_CP103731:4151678-4151896 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14810.93 Da        Isoelectric Point: 7.2048

>AT208795 WP_010792483.1 NZ_CP077978:c154648-154253 [Pseudomonas aeruginosa]
MASKAVLELRPTNFAGLMDALKEVNRVMFSPERFLKVFSMDQQTLALRAHVHRNTVRNAPESEKVQAYIRDSVKVLRAVT
DMGTDVTNAIFWFKNEPLSTFNYKTAEEVVSEGKTEQLIAFLQSWEAGAQG

Download         Length: 396 bp

>AT208795 NZ_CP103731:4151277-4151651 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A212BIN6

Antitoxin

Source ID Structure
AlphaFold DB A0A2I0C738

References