Detailed information of TA system TA020879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 261079..261693
• Replicon: chromosome
• Accession: NC_004578
• Organism: Pseudomonas syringae pv. tomato str. DC3000

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: Q88AZ2
• Locus tag: PSPTO_RS01275
• Protein ID: WP_005768484.1
• Coordinates: 261343..261693 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: Q88AZ3
• Locus tag: PSPTO_RS01270
• Protein ID: WP_007246542.1
• Coordinates: 261079..261330 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSPTO_RS01245	256145..256588	-	444	WP_172679536.1	ATP-dependent zinc protease	-
PSPTO_RS01250	256638..257420	-	783	WP_005768488.1	EAL domain-containing protein	-
PSPTO_RS01255	257588..257995	+	408	WP_003377907.1	RNA-binding protein S4	-
PSPTO_RS01260	258156..259058	+	903	WP_005613652.1	Hsp33 family molecular chaperone HslO	-
PSPTO_RS01265	259238..260782	+	1545	WP_005613653.1	phosphoenolpyruvate carboxykinase	-
PSPTO_RS01270	261079..261330	+	252	WP_007246542.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PSPTO_RS01275	261343..261693	+	351	WP_005768484.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSPTO_RS01280	261996..262448	-	453	WP_024666863.1	hypothetical protein	-
PSPTO_RS01285	262839..263453	-	615	WP_046463847.1	hypothetical protein	-
PSPTO_RS01290	263550..264257	-	708	WP_046463848.1	hypothetical protein	-
PSPTO_RS01295	264386..264835	-	450	WP_074843309.1	hypothetical protein	-
PSPTO_RS01300	265284..265997	-	714	WP_011103045.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-101)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13110.05 Da
• Isoelectric Point: 4.3176

>T20879 WP_005768484.1 NC_004578:261343-261693 [Pseudomonas syringae pv. tomato str. DC3000]
MNMFALRFTDVAQQSLEDQVEHLAVYQGFSPAAQRIDTLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNADGYRIFY
EVRETDDINVIVIVLVLGGKQSVEQALIRYCLLQPI

Nucleotide

• Length: 351 bp

>T20879 NC_004578:261343-261693 [Pseudomonas syringae pv. tomato str. DC3000]
ATGAATATGTTTGCACTGCGCTTTACCGATGTTGCCCAGCAAAGCCTCGAAGATCAGGTTGAACACCTGGCGGTCTATCA
GGGATTCTCTCCCGCTGCTCAGCGCATAGACACCCTGATTGATGCCATTCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCTCAATTGAGTGAGCTGGGCGTACTGCACTATCGTGAGTTGAACGCTGACGGATACCGGATCTTTTAC
GAGGTGAGGGAGACAGATGATATTAATGTCATCGTCATAGTGCTCGTATTAGGTGGTAAACAAAGTGTTGAACAGGCACT
AATCCGCTATTGTCTGCTTCAGCCTATTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9474.83 Da
• Isoelectric Point: 9.5019

>AT20879 WP_007246542.1 NC_004578:261079-261330 [Pseudomonas syringae pv. tomato str. DC3000]
MRVETISYLKRNAADLPLDEPLIVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSADELKARLSRRFA
HKE

Nucleotide

• Length: 252 bp

>AT20879 NC_004578:261079-261330 [Pseudomonas syringae pv. tomato str. DC3000]
ATGCGAGTTGAAACAATAAGCTATTTGAAACGCAATGCAGCCGATCTGCCTCTCGATGAGCCACTGATTGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCAGATCGTAAGCGTCGCGATGAGTCCATTGCCTTGGTGAAGCTCTTGG
CAATCAGTTCCCGCGAGTACTCCCAAGGCAAGCACTGTTCTGCTGACGAACTTAAAGCCCGATTGAGCAGGAGGTTTGCC
CACAAAGAATAA

Structures

Toxin

• AlphaFold DB: Q88AZ2

Antitoxin

• AlphaFold DB: Q88AZ3