Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RelE-RHH |
| Location | 9445..9995 | Replicon | plasmid p2 |
| Accession | NZ_CP077973 | ||
| Organism | Pseudomonas aeruginosa strain ZPPH33 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | - |
| Locus tag | KUU78_RS31305 | Protein ID | WP_019727125.1 |
| Coordinates | 9445..9720 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | A0A6B1YL09 |
| Locus tag | KUU78_RS31310 | Protein ID | WP_019727126.1 |
| Coordinates | 9720..9995 (-) | Length | 92 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KUU78_RS31260 (KUU78_31245) | 4570..4869 | + | 300 | WP_196994194.1 | hypothetical protein | - |
| KUU78_RS31265 (KUU78_31250) | 4982..5188 | + | 207 | WP_152996423.1 | hypothetical protein | - |
| KUU78_RS31270 (KUU78_31255) | 5215..5469 | + | 255 | WP_196994195.1 | hypothetical protein | - |
| KUU78_RS31275 (KUU78_31260) | 6055..6732 | + | 678 | WP_034018017.1 | SOS response-associated peptidase | - |
| KUU78_RS31280 (KUU78_31265) | 6841..7143 | + | 303 | WP_023131919.1 | anti-CRISPR protein AcrE1 | - |
| KUU78_RS31285 (KUU78_31270) | 7166..7588 | + | 423 | WP_196994196.1 | anti-CRISPR protein AcrF3 | - |
| KUU78_RS31290 (KUU78_31275) | 7601..7840 | + | 240 | WP_196994197.1 | anti-CRISPR protein | - |
| KUU78_RS32340 | 7837..8040 | + | 204 | WP_031757130.1 | helix-turn-helix transcriptional regulator | - |
| KUU78_RS31295 (KUU78_31280) | 8136..8456 | + | 321 | WP_196994198.1 | hypothetical protein | - |
| KUU78_RS31300 (KUU78_31285) | 8938..9354 | + | 417 | WP_196994199.1 | hypothetical protein | - |
| KUU78_RS31305 (KUU78_31290) | 9445..9720 | - | 276 | WP_019727125.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| KUU78_RS31310 (KUU78_31295) | 9720..9995 | - | 276 | WP_019727126.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| KUU78_RS31315 (KUU78_31300) | 10270..10656 | + | 387 | WP_019727127.1 | hypothetical protein | - |
| KUU78_RS31320 (KUU78_31305) | 10717..11010 | + | 294 | WP_019727128.1 | hypothetical protein | - |
| KUU78_RS31325 (KUU78_31310) | 11334..11642 | + | 309 | WP_019727129.1 | TrfB-related DNA-binding protein | - |
| KUU78_RS31330 (KUU78_31315) | 12292..12744 | + | 453 | WP_019727132.1 | DNA repair protein RadC | - |
| KUU78_RS31335 (KUU78_31320) | 13182..13430 | + | 249 | WP_058130600.1 | hypothetical protein | - |
| KUU78_RS31340 (KUU78_31325) | 13480..13731 | - | 252 | WP_058130599.1 | hypothetical protein | - |
| KUU78_RS31345 (KUU78_31330) | 13790..14074 | + | 285 | WP_019727136.1 | hypothetical protein | - |
| KUU78_RS31350 (KUU78_31335) | 14117..14494 | + | 378 | WP_019727137.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..53593 | 53593 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10779.36 Da Isoelectric Point: 7.1659
>T208789 WP_019727125.1 NZ_CP077973:c9720-9445 [Pseudomonas aeruginosa]
MELKWTSKGLSDVARLYEFLATVNREAAARTVQQLTSAPTALLTNPRLGERLEEFEPRDVRRILIGHYEMRYEILDSCIY
VLRLWHTREDR
MELKWTSKGLSDVARLYEFLATVNREAAARTVQQLTSAPTALLTNPRLGERLEEFEPRDVRRILIGHYEMRYEILDSCIY
VLRLWHTREDR
Download Length: 276 bp
>T208789 NZ_CP103731:324785-325153 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA
Antitoxin
Download Length: 92 a.a. Molecular weight: 10142.32 Da Isoelectric Point: 4.8433
>AT208789 WP_019727126.1 NZ_CP077973:c9995-9720 [Pseudomonas aeruginosa]
MSTAAKPTRTVTAHVPVDLAERVDAIAERLERSKNWIVRQALSAWIDQEDERSRLTREALDDVDAGRVVDHQAVQAWAES
LGTDSPLPVPR
MSTAAKPTRTVTAHVPVDLAERVDAIAERLERSKNWIVRQALSAWIDQEDERSRLTREALDDVDAGRVVDHQAVQAWAES
LGTDSPLPVPR
Download Length: 276 bp
>AT208789 NZ_CP103731:324567-324788 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|