Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4362263..4362858 Replicon chromosome
Accession NZ_CP077969
Organism Escherichia coli strain JM83

Toxin (Protein)


Gene name chpB Uniprot ID U9XNP6
Locus tag KTJ86_RS21065 Protein ID WP_000239577.1
Coordinates 4362508..4362858 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag KTJ86_RS21060 Protein ID WP_001223208.1
Coordinates 4362263..4362514 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTJ86_RS21050 (4357928) 4357928..4361707 + 3780 WP_000060911.1 autotransporter assembly complex protein TamB -
KTJ86_RS21055 (4361710) 4361710..4362051 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
KTJ86_RS21060 (4362263) 4362263..4362514 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
KTJ86_RS21065 (4362508) 4362508..4362858 + 351 WP_000239577.1 endoribonuclease toxin ChpB Toxin
KTJ86_RS21070 (4362938) 4362938..4363468 - 531 WP_000055075.1 inorganic diphosphatase -
KTJ86_RS21075 (4363778) 4363778..4364734 + 957 WP_000265913.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
KTJ86_RS21080 (4364874) 4364874..4366376 + 1503 WP_000205805.1 sugar ABC transporter ATP-binding protein -
KTJ86_RS21085 (4366390) 4366390..4367412 + 1023 WP_001313531.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12492.40 Da        Isoelectric Point: 5.5572

>T208776 WP_000239577.1 NZ_CP077969:4362508-4362858 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T208776 NZ_CP103728:11347-11763 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT208776 WP_001223208.1 NZ_CP077969:4362263-4362514 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT208776 NZ_CP103728:11120-11350 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAAGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XNP6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References