Detailed information of TA system TA020877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2564087..2564649
• Replicon: chromosome
• Accession: NC_004567
• Organism: Lactobacillus plantarum WCFS1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F9URY0
• Locus tag: LP_RS12175
• Protein ID: WP_011101950.1
• Coordinates: 2564087..2564434 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: LP_RS12180
• Protein ID: WP_063851726.1
• Coordinates: 2564434..2564649 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LP_RS12145	2559329..2560186	-	858	WP_011101946.1	SDR family oxidoreductase	-
LP_RS12150	2560627..2560848	-	222	WP_011101947.1	hypothetical protein	-
LP_RS12155	2561104..2561547	-	444	WP_003642165.1	universal stress protein	-
LP_RS12160	2561619..2562188	-	570	WP_003642166.1	sugar O-acetyltransferase	-
LP_RS12165	2562285..2562968	+	684	WP_011101948.1	YjjG family noncanonical pyrimidine nucleotidase	-
LP_RS12170	2563118..2563633	-	516	WP_011101949.1	hypothetical protein	-
LP_RS12175	2564087..2564434	-	348	WP_011101950.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LP_RS12180	2564434..2564649	-	216	WP_063851726.1	hypothetical protein	Antitoxin
LP_RS12185	2565416..2565955	-	540	WP_011101952.1	hypothetical protein	-
LP_RS12190	2566782..2567375	-	594	WP_011101953.1	NAD(P)-binding domain-containing protein	-
LP_RS12195	2567524..2568696	-	1173	WP_011101954.1	pyridoxal phosphate-dependent aminotransferase	-
LP_RS12200	2568686..2569552	-	867	WP_011101955.1	DUF3737 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13274.06 Da
• Isoelectric Point: 9.5016

>T20877 WP_011101950.1 NC_004567:c2564434-2564087 [Lactobacillus plantarum WCFS1]
MTYLPKQKDIIWIDFDPQRGREIKKRRPAVVLSSNLYTQNTGFVIVSPITSTMRDLPGYFSLNGYNTHGQIAAAQIYSFD
ATPRAGRSITYIETMRSADFYHVAQTVYYNFDFPF

Nucleotide

• Length: 348 bp

>T20877 NC_004567:c2564434-2564087 [Lactobacillus plantarum WCFS1]
ATGACTTATTTACCTAAACAAAAAGACATCATCTGGATTGATTTTGACCCACAACGTGGCCGCGAAATAAAGAAACGTCG
GCCAGCTGTAGTTCTTAGTAGTAATCTCTACACTCAGAACACCGGGTTTGTAATTGTTTCACCAATTACGTCTACAATGC
GTGATTTGCCCGGCTACTTTAGTCTTAATGGTTACAATACCCACGGACAAATTGCTGCTGCCCAAATTTATTCGTTTGAT
GCGACCCCCAGAGCAGGACGGAGTATCACATACATCGAAACCATGCGCAGTGCCGATTTTTATCACGTCGCGCAAACGGT
GTATTACAACTTCGACTTTCCATTCTAA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8032.08 Da
• Isoelectric Point: 8.5318

>AT20877 WP_063851726.1 NC_004567:c2564649-2564434 [Lactobacillus plantarum WCFS1]
IEVKTVSRGNSLALSLPKDGRFKKGQRWLLIPSSDGESYTLVPRIENPYAGSKPKRPMTEAWSDVDWNEVE

Nucleotide

• Length: 216 bp

>AT20877 NC_004567:c2564649-2564434 [Lactobacillus plantarum WCFS1]
ATTGAGGTAAAGACAGTTAGTCGTGGTAATTCATTGGCATTAAGTCTACCTAAAGACGGTCGTTTTAAGAAAGGACAACG
ATGGTTACTCATCCCATCAAGTGATGGTGAGTCATATACACTCGTACCACGTATCGAGAATCCTTATGCCGGCTCCAAGC
CGAAACGGCCAATGACTGAAGCATGGTCCGATGTCGACTGGAATGAGGTCGAGTAA

Structures

Toxin

• AlphaFold DB: F9URY0