208768

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2678510..2679947 Replicon chromosome
Accession NZ_CP077969
Organism Escherichia coli strain JM83

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag KTJ86_RS13045 Protein ID WP_000155570.1
Coordinates 2678510..2679583 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag KTJ86_RS13050 Protein ID WP_000461704.1
Coordinates 2679576..2679947 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KTJ86_RS13030 (2673943) 2673943..2676132 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
KTJ86_RS13035 (2676129) 2676129..2677745 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
KTJ86_RS13040 (2678105) 2678105..2678368 - 264 WP_000502050.1 DUF1524 domain-containing protein -
KTJ86_RS13045 (2678510) 2678510..2679583 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
KTJ86_RS13050 (2679576) 2679576..2679947 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
KTJ86_RS13055 (2680302) 2680302..2681165 + 864 WP_000787597.1 GTPase family protein -
KTJ86_RS13060 (2681257) 2681257..2682078 + 822 WP_000197391.1 DUF932 domain-containing protein -
KTJ86_RS13065 (2682295) 2682295..2682996 + 702 WP_000189409.1 WYL domain-containing protein -
KTJ86_RS13070 (2683037) 2683037..2683273 + 237 WP_001144029.1 protein YpjK -
KTJ86_RS13075 (2683273) 2683273..2683716 + 444 WP_000824222.1 lipoprotein YfjS -
KTJ86_RS13080 (2683740) 2683740..2684207 + 468 WP_000702495.1 protein YfjT -
KTJ86_RS22055 (2684594) 2684594..2684746 - 153 Protein_2561 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T208768 WP_000155570.1 NZ_CP077969:2678510-2679583 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T208768 NZ_CP103727:786309-786719 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT208768 WP_000461704.1 NZ_CP077969:2679576-2679947 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT208768 NZ_CP103727:786062-786328 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References