Detailed information of TA system TA208766

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1995149..1995980
• Replicon: chromosome
• Accession: NZ_CP077969
• Organism: Escherichia coli strain JM83

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: KTJ86_RS09870
• Protein ID: WP_000854814.1
• Coordinates: 1995606..1995980 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: KTJ86_RS09865
• Protein ID: WP_001285584.1
• Coordinates: 1995149..1995517 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTJ86_RS09850 (1992816)	1992816..1994348	+	1533	WP_001350525.1	protein YeeR	-
KTJ86_RS09855 (1994345)	1994345..1994791	+	447	WP_000187523.1	RadC family protein	-
KTJ86_RS09860 (1994854)	1994854..1995075	+	222	WP_000692323.1	DUF987 domain-containing protein	-
KTJ86_RS09865 (1995149)	1995149..1995517	+	369	WP_001285584.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
KTJ86_RS09870 (1995606)	1995606..1995980	+	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KTJ86_RS09875 (1995977)	1995977..1996171	+	195	WP_000988600.1	DUF5983 family protein	-
KTJ86_RS09880 (1996217)	1996217..1996297	+	81	Protein_1932	hypothetical protein	-
KTJ86_RS09885 (1996586)	1996586..1996714	-	129	Protein_1933	transposase domain-containing protein	-
KTJ86_RS09890 (1996834)	1996834..1996968	+	135	WP_001297944.1	EutP/PduV family microcompartment system protein	-
KTJ86_RS09895 (1997069)	1997069..1997398	-	330	WP_000450409.1	DUF496 family protein	-
KTJ86_RS09900 (1997570)	1997570..1998628	-	1059	WP_001200891.1	FUSC family protein	-
KTJ86_RS09905 (1998826)	1998826..1999299	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
KTJ86_RS09910 (1999418)	1999418..2000584	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T208766 WP_000854814.1 NZ_CP077969:1995606-1995980 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T208766 NZ_CP103727:329148-329516 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT208766 WP_001285584.1 NZ_CP077969:1995149-1995517 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT208766 NZ_CP103727:328930-329151 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A1M2E8G6