Detailed information of TA system TA020875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1567642..1568228
• Replicon: chromosome
• Accession: NC_004556
• Organism: Xylella fastidiosa Temecula1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q87BV5
• Locus tag: PD_RS11980
• Protein ID: WP_004089517.1
• Coordinates: 1567974..1568228 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B2I6C9
• Locus tag: PD_RS07095
• Protein ID: WP_004085025.1
• Coordinates: 1567642..1567977 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PD_RS07065	1563241..1564095	-	855	WP_011098055.1	iron-sulfur cluster carrier protein ApbC	-
PD_RS07070	1564587..1565360	+	774	WP_004089528.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
PD_RS07075	1565357..1565821	+	465	WP_011098056.1	low molecular weight phosphotyrosine protein phosphatase	-
PD_RS07080	1565808..1566521	-	714	WP_011098057.1	site-specific DNA-methyltransferase	-
PD_RS07085	1566872..1567153	+	282	WP_011098058.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PD_RS07090	1567163..1567462	+	300	WP_004089519.1	HigA family addiction module antidote protein	-
PD_RS07095	1567642..1567977	-	336	WP_004085025.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PD_RS11980	1567974..1568228	-	255	WP_004089517.1	type II toxin-antitoxin system HicA family toxin	Toxin
PD_RS07100	1568281..1568979	-	699	WP_011098059.1	hypothetical protein	-
PD_RS07105	1569108..1569569	-	462	WP_011098060.1	hypothetical protein	-
PD_RS07110	1569566..1570330	-	765	WP_004090689.1	hypothetical protein	-
PD_RS07115	1570236..1571351	-	1116	WP_011098062.1	YdaU family protein	-
PD_RS07120	1571348..1571647	-	300	WP_080513037.1	hypothetical protein	-
PD_RS11985	1571646..1572125	+	480	WP_011098064.1	conjugal transfer protein	-
PD_RS07130	1572131..1572736	+	606	WP_004091108.1	conjugal transfer protein TrbN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-80)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9639.14 Da
• Isoelectric Point: 11.2766

>T20875 WP_004089517.1 NC_004556:c1568228-1567974 [Xylella fastidiosa Temecula1]
MKRKHAKTLSAIYTRPVSANIQWRDIEALFVELGAKVEEREGSRMLVRLFGERRIFHRPHPSPNTDKGAVESIRAWLKSN
GVTP

Nucleotide

• Length: 255 bp

>T20875 NC_004556:c1568228-1567974 [Xylella fastidiosa Temecula1]
ATGAAGCGTAAGCACGCTAAAACTCTCAGCGCTATATACACACGCCCTGTTTCGGCCAACATTCAATGGCGCGATATTGA
AGCGCTGTTCGTGGAGCTTGGAGCGAAAGTAGAAGAACGTGAAGGTTCTCGTATGTTGGTGCGACTGTTCGGTGAACGTC
GGATATTCCACCGGCCCCACCCTTCTCCTAACACTGACAAGGGTGCTGTTGAATCAATCCGAGCTTGGTTGAAATCGAAC
GGAGTTACACCATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12324.07 Da
• Isoelectric Point: 5.6556

>AT20875 WP_004085025.1 NC_004556:c1567977-1567642 [Xylella fastidiosa Temecula1]
MKNIMTIESFKAIITYDPEIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACKEDSVEPRKCYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEVHT

Nucleotide

• Length: 336 bp

>AT20875 NC_004556:c1567977-1567642 [Xylella fastidiosa Temecula1]
ATGAAGAACATCATGACTATTGAGAGCTTTAAAGCCATCATTACCTATGATCCGGAGATCGACATGTTTCGCGGTGAATT
TGTGGGTATCAATGGTGGTGCTGATTTCTATGCAAAAGACCTTAAGGGTCTTCGTCGAGAAGGAGCTATTTCTCTGAAGG
TATTTTTGGAAGCCTGCAAAGAAGATAGCGTGGAACCACGTAAATGCTACTCAGGGAAGTTCAATGCAAGGATTTCTCCT
GAACTGCATGCTTTGGCTTCTGAAGCCGCAGCAGCACAGGGCATCAGTTTGAACCAATTTGTAGAACAAGCGATTCAGCA
TGAAGTCCACACCTGA

Structures

Toxin

• AlphaFold DB: Q87BV5

Antitoxin

• AlphaFold DB: A0A060HAK5