Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 27092..27674 Replicon plasmid p6Pmi283-2
Accession NZ_CP077965
Organism Proteus mirabilis strain 6Pmi283

Toxin (Protein)


Gene name PumA Uniprot ID B1VJ82
Locus tag KPK64_RS19385 Protein ID WP_012368903.1
Coordinates 27378..27674 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID B1VJ81
Locus tag KPK64_RS19380 Protein ID WP_012368902.1
Coordinates 27092..27376 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPK64_RS19335 (KPK64_03886) 22363..22644 + 282 WP_113355595.1 CopG family transcriptional regulator -
KPK64_RS19340 (KPK64_03887) 22700..22876 + 177 WP_218211683.1 hypothetical protein -
KPK64_RS19345 (KPK64_03888) 23137..23382 + 246 WP_113355596.1 hypothetical protein -
KPK64_RS19350 (KPK64_03889) 23382..23711 + 330 WP_113355598.1 hypothetical protein -
KPK64_RS19355 (KPK64_03890) 23802..24326 + 525 WP_255346644.1 restriction endonuclease -
KPK64_RS19360 (KPK64_03891) 24303..24659 - 357 WP_073546978.1 DNA distortion polypeptide 3 -
KPK64_RS19365 (KPK64_03892) 24669..25829 - 1161 WP_113355603.1 MobP1 family relaxase -
KPK64_RS19370 (KPK64_03893) 25837..26385 - 549 WP_218211685.1 DNA distortion polypeptide 1 -
KPK64_RS19375 26705..26860 + 156 WP_218211687.1 hypothetical protein -
KPK64_RS19380 (KPK64_03894) 27092..27376 - 285 WP_012368902.1 putative addiction module antidote protein Antitoxin
KPK64_RS19385 (KPK64_03895) 27378..27674 - 297 WP_012368903.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KPK64_RS19790 (KPK64_03896) 27794..27928 - 135 WP_012368904.1 hypothetical protein -
KPK64_RS19390 (KPK64_03897) 28004..28348 - 345 WP_012368905.1 hypothetical protein -
KPK64_RS19395 (KPK64_03898) 28412..28669 - 258 WP_113355607.1 hypothetical protein -
KPK64_RS19400 (KPK64_03899) 28674..28856 - 183 WP_012368908.1 hypothetical protein -
KPK64_RS19405 (KPK64_03900) 29229..29459 - 231 WP_012368910.1 transcriptional regulator -
KPK64_RS19410 (KPK64_03901) 29449..29715 - 267 WP_218211689.1 hypothetical protein -
KPK64_RS19415 (KPK64_03902) 29737..30288 - 552 WP_092514124.1 hypothetical protein -
KPK64_RS19420 (KPK64_03903) 30358..30564 - 207 WP_012368913.1 hypothetical protein -
KPK64_RS19425 (KPK64_03904) 30619..31143 - 525 WP_012368914.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..32132 32132


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Uncharacterized protein HI1420

(3-90)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11325.21 Da        Isoelectric Point: 10.2910

>T208741 WP_012368903.1 NZ_CP077965:c27674-27378 [Proteus mirabilis]
MIEIRQTEEVKKWLKGLKDKIAKAKILTRIERMKEGNYGDVEPIGDGYSELRIHQGKGYRVYFANRNNEIILLLCGGDKT
TQQADIKKAKQIAKKWGF

Download         Length: 297 bp

>T208741 NZ_CP103718:c3140438-3140187 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10302.61 Da        Isoelectric Point: 4.6286

>AT208741 WP_012368902.1 NZ_CP077965:c27376-27092 [Proteus mirabilis]
MKTKPELFDVVDYLEDEEDIQAYLNAALDEDDESYFLAALGNIARARNISQLSKETGMSREGIYKALSGTGNPSFKTISK
ISKALGLKIHFAGA

Download         Length: 285 bp

>AT208741 NZ_CP103718:c3140190-3139936 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I3XCB7

Antitoxin

Source ID Structure
AlphaFold DB A0A1I3XCJ1

References