Detailed information of TA system TA020874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1379517..1380122
• Replicon: chromosome
• Accession: NC_004556
• Organism: Xylella fastidiosa Temecula1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: PD_RS06210
• Protein ID: WP_004089252.1
• Coordinates: 1379517..1379813 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: PD_RS06215
• Protein ID: WP_004089254.1
• Coordinates: 1379817..1380122 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PD_RS06185	1374682..1375659	+	978	WP_004087353.1	hypothetical protein	-
PD_RS06190	1375918..1376364	+	447	WP_004087355.1	hypothetical protein	-
PD_RS06195	1376468..1377712	-	1245	WP_011097983.1	phage tail protein	-
PD_RS06200	1377716..1378276	-	561	WP_011097869.1	DUF2612 domain-containing protein	-
PD_RS06205	1378273..1379418	-	1146	WP_011097984.1	baseplate J/gp47 family protein	-
PD_RS06210	1379517..1379813	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PD_RS06215	1379817..1380122	+	306	WP_004089254.1	putative addiction module antidote protein	Antitoxin
PD_RS06220	1380133..1380486	-	354	WP_011097986.1	hypothetical protein	-
PD_RS06225	1380483..1381124	-	642	WP_004090764.1	hypothetical protein	-
PD_RS06230	1381121..1381951	-	831	WP_004090766.1	hypothetical protein	-
PD_RS06235	1381948..1382265	-	318	WP_011097873.1	hypothetical protein	-
PD_RS06240	1382265..1383035	-	771	WP_004090769.1	hypothetical protein	-
PD_RS06245	1383083..1383373	+	291	WP_004087089.1	hypothetical protein	-
PD_RS06250	1383357..1383623	+	267	WP_011097988.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1376468..1394767	18299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T20874 WP_004089252.1 NC_004556:1379517-1379813 [Xylella fastidiosa Temecula1]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T20874 NC_004556:1379517-1379813 [Xylella fastidiosa Temecula1]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATCTCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.37 Da
• Isoelectric Point: 5.0941

>AT20874 WP_004089254.1 NC_004556:1379817-1380122 [Xylella fastidiosa Temecula1]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSNDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT20874 NC_004556:1379817-1380122 [Xylella fastidiosa Temecula1]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAGCTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A060HBK9