Detailed information of TA system TA208739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3939780..3941351
• Replicon: chromosome
• Accession: NZ_CP077963
• Organism: Proteus mirabilis strain 6Pmi283

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KPK64_RS17980
• Protein ID: WP_218211429.1
• Coordinates: 3939780..3941093 (-)
• Length: 438 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: B4F0T9
• Locus tag: KPK64_RS17985
• Protein ID: WP_004249771.1
• Coordinates: 3941094..3941351 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPK64_RS17960 (KPK64_03618)	3934949..3935752	+	804	WP_004249775.1	energy transducer TonB	-
KPK64_RS17965 (KPK64_03619)	3935806..3937767	-	1962	WP_196572567.1	insecticidal delta-endotoxin Cry8Ea1 family protein	-
KPK64_RS17970 (KPK64_03620)	3937799..3938728	-	930	WP_004246518.1	metallophosphoesterase	-
KPK64_RS17975 (KPK64_03621)	3938913..3939377	-	465	WP_004249773.1	DUF1097 domain-containing protein	-
KPK64_RS17980 (KPK64_03622)	3939780..3941093	-	1314	WP_218211429.1	type II toxin-antitoxin system HipA family toxin	Toxin
KPK64_RS17985 (KPK64_03623)	3941094..3941351	-	258	WP_004249771.1	helix-turn-helix domain-containing protein	Antitoxin
KPK64_RS17990 (KPK64_03624)	3941500..3942864	-	1365	WP_196549150.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
KPK64_RS17995 (KPK64_03625)	3943002..3944642	-	1641	WP_004246512.1	membrane protein insertase YidC	-
KPK64_RS18000	3944645..3944905	-	261	WP_012368639.1	membrane protein insertion efficiency factor YidD	-
KPK64_RS18005 (KPK64_03626)	3944869..3945228	-	360	WP_004246510.1	ribonuclease P protein component	-
KPK64_RS18010 (KPK64_03627)	3945246..3945389	-	144	WP_004246509.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 438 a.a.
• Molecular weight: 49706.81 Da
• Isoelectric Point: 6.6587

>T208739 WP_218211429.1 NZ_CP077963:c3941093-3939780 [Proteus mirabilis]
MASLYVYMNEYYVGIFTKKSSGAHSFQYTEDWVNQPGSRPISLSMPLQLQEYQGDCVYNFFDNLLPDNTEIRNRIVSRYN
ANSNQPFDLLAAIGADTVGALRLLPSGSTPADIKKIEYQKLNDTELEKILLGYKSNTPLGMLKEYTDFRISIAGAQEKTA
LLLKDNHWCVPHNNTPTTHIIKLPIGVIESHSYTMDLSNSVENEYLCTLIAKEFDLPVPHCSIIKTKNVKALAVERFDRK
YSSDKSWIIRLPQEDFCQILNIPSALKYENEGGPGIIDIMKYLLGSSNADQDRFHFMKAQVLFWLLAATDGHAKNFSVFI
NQEGRFHLTPLYDIMSIYPNFGGRGLNPRDAKLAMGLKASRGKKYAIEQIFPRHFYQTAEAVGFDPSLMSDILRYFYQEM
DDVIQRVKQQLPEDFPSEISNTILDGMKARAVRLANI

Nucleotide

• Length: 1314 bp

>T208739 NZ_CP103718:c2933881-2933778 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9878.43 Da
• Isoelectric Point: 10.0139

>AT208739 WP_004249771.1 NZ_CP077963:c3941351-3941094 [Proteus mirabilis]
MIIINAYQLSVHLKDVRLTKKLSQSKVAQKVGIRQDTVSNFELNPNSTKLETFFKLLSALNLEMEIHPKDAKNNLAHKSE
WKEEW

Nucleotide

• Length: 258 bp

>AT208739 NZ_CP103718:2933740-2934011 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: B4F0T9