Detailed information of TA system TA208738

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 3654961..3655660
• Replicon: chromosome
• Accession: NZ_CP077963
• Organism: Proteus mirabilis strain 6Pmi283

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B4EZ96
• Locus tag: KPK64_RS16730
• Protein ID: WP_004249093.1
• Coordinates: 3654961..3655347 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KPK64_RS16735
• Protein ID: WP_107336998.1
• Coordinates: 3655340..3655660 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPK64_RS16715 (KPK64_03361)	3650980..3651561	-	582	WP_012368524.1	DNA-3-methyladenine glycosylase I	-
KPK64_RS16720 (KPK64_03362)	3651812..3652717	+	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
KPK64_RS16725 (KPK64_03363)	3652727..3654799	+	2073	WP_017827114.1	glycine--tRNA ligase subunit beta	-
KPK64_RS16730 (KPK64_03364)	3654961..3655347	+	387	WP_004249093.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KPK64_RS16735 (KPK64_03365)	3655340..3655660	+	321	WP_107336998.1	helix-turn-helix domain-containing protein	Antitoxin
KPK64_RS16740 (KPK64_03366)	3655709..3656143	-	435	WP_004246827.1	hypothetical protein	-
KPK64_RS16745 (KPK64_03367)	3656136..3657020	-	885	WP_012368526.1	endonuclease/exonuclease/phosphatase family protein	-
KPK64_RS16750 (KPK64_03368)	3657237..3658523	-	1287	WP_049196833.1	DUF3748 domain-containing protein	-
KPK64_RS16755 (KPK64_03369)	3658660..3659277	+	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
KPK64_RS16760 (KPK64_03370)	3659579..3660202	+	624	WP_004246821.1	guanylate kinase	-
KPK64_RS16765 (KPK64_03371)	3660257..3660532	+	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14294.64 Da
• Isoelectric Point: 10.0642

>T208738 WP_004249093.1 NZ_CP077963:3654961-3655347 [Proteus mirabilis]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNANQNKIDKMIALGLLTEVKYD

Nucleotide

• Length: 387 bp

>T208738 NZ_CP103718:c2647577-2647290 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12145.25 Da
• Isoelectric Point: 10.5680

>AT208738 WP_107336998.1 NZ_CP077963:3655340-3655660 [Proteus mirabilis]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKRPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT208738 NZ_CP103718:c2647816-2647577 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: B4EZ96