Detailed information of TA system TA208737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3156102..3157123
• Replicon: chromosome
• Accession: NZ_CP077963
• Organism: Proteus mirabilis strain 6Pmi283

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A8E4FRK4
• Locus tag: KPK64_RS14615
• Protein ID: WP_017827532.1
• Coordinates: 3156554..3157123 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: KPK64_RS14610
• Protein ID: WP_004245528.1
• Coordinates: 3156102..3156554 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPK64_RS14590 (KPK64_02935)	3151888..3152418	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
KPK64_RS14595 (KPK64_02936)	3152735..3153700	+	966	WP_049195511.1	thiamine ABC transporter substrate binding subunit	-
KPK64_RS14600 (KPK64_02937)	3153700..3155280	+	1581	WP_196549172.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
KPK64_RS14605 (KPK64_02938)	3155273..3155974	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
KPK64_RS14610 (KPK64_02939)	3156102..3156554	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
KPK64_RS14615 (KPK64_02940)	3156554..3157123	+	570	WP_017827532.1	PIN domain-containing protein	Toxin
KPK64_RS14620 (KPK64_02941)	3157239..3159593	+	2355	WP_012368319.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21601.08 Da
• Isoelectric Point: 7.0180

>T208737 WP_017827532.1 NZ_CP077963:3156554-3157123 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKDPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T208737 NZ_CP103718:c2554366-2554190 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT208737 WP_004245528.1 NZ_CP077963:3156102-3156554 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT208737 NZ_CP103718:c2554144-2553728 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: B4F2H3