Detailed information of TA system TA208733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 9329..9905
• Replicon: plasmid pWHA07
• Accession: NZ_CP077961
• Organism: Mammaliicoccus sciuri strain WHA07

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KTR69_RS15575
• Protein ID: WP_185160639.1
• Coordinates: 9329..9679 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KTR69_RS15580
• Protein ID: WP_204252311.1
• Coordinates: 9672..9905 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTR69_RS15555	5381..6520	-	1140	WP_218697531.1	arginase family protein	-
KTR69_RS15560	6517..7680	-	1164	WP_204252313.1	hypothetical protein	-
KTR69_RS15565	8579..8791	-	213	WP_218697533.1	hypothetical protein	-
KTR69_RS15570	8933..9241	-	309	WP_218697534.1	hypothetical protein	-
KTR69_RS15575	9329..9679	-	351	WP_185160639.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KTR69_RS15580	9672..9905	-	234	WP_204252311.1	AbrB family transcriptional regulator	Antitoxin
KTR69_RS15585	11391..11837	-	447	WP_204252310.1	hypothetical protein	-
KTR69_RS15930	12272..12545	+	274	Protein_12	MazG-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..58992	58992

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13347.34 Da
• Isoelectric Point: 10.0739

>T208733 WP_185160639.1 NZ_CP077961:c9679-9329 [Mammaliicoccus sciuri]
MNSISQGTLIYIDFEPSKGSEIKKRRPAIVISRDEYNLASNLVIVCPITSTDKDRPYFVPINNKNLKSNSKVNTKQVYSL
DYTERAGRNIQIIGKITNKELMNIAQHFLMNFNFNF

Nucleotide

• Length: 351 bp

>T208733 NZ_CP103718:c2209715-2209608 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8679.68 Da
• Isoelectric Point: 4.2220

>AT208733 WP_204252311.1 NZ_CP077961:c9905-9672 [Mammaliicoccus sciuri]
MDKVKTRKQGNAVMVTIAKKFNVPEGQEFYITKESDGTISLIPKIQDYYKEVTAGEFIDEEDSLAQNFTTAGNELDE

Nucleotide

• Length: 234 bp

>AT208733 NZ_CP103718:2209763-2209829 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC