Detailed information of TA system TA208729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 3298881..3299509
• Replicon: chromosome
• Accession: NZ_CP077942
• Organism: Leptospira interrogans serovar Bataviae strain D64

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8F7E4
• Locus tag: GR153_RS13715
• Protein ID: WP_000281042.1
• Coordinates: 3299111..3299509 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A868A7V8
• Locus tag: GR153_RS13710
• Protein ID: WP_001192479.1
• Coordinates: 3298881..3299111 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GR153_RS13690 (GR153_013700)	3294179..3295162	-	984	WP_020783280.1	hypothetical protein	-
GR153_RS13695 (GR153_013705)	3295378..3296067	+	690	WP_000590461.1	SH3 domain-containing protein	-
GR153_RS13700 (GR153_013710)	3296339..3296641	+	303	WP_001092687.1	hypothetical protein	-
GR153_RS13705 (GR153_013715)	3296687..3298525	+	1839	WP_001015573.1	P-loop NTPase fold protein	-
GR153_RS13710 (GR153_013720)	3298881..3299111	+	231	WP_001192479.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GR153_RS13715 (GR153_013725)	3299111..3299509	+	399	WP_000281042.1	type II toxin-antitoxin system VapC family toxin	Toxin
GR153_RS13720 (GR153_013730)	3299607..3299918	+	312	WP_000479561.1	hypothetical protein	-
GR153_RS13725 (GR153_013735)	3299929..3300156	+	228	WP_000130601.1	hypothetical protein	-
GR153_RS13730 (GR153_013740)	3300298..3301378	+	1081	Protein_2727	hypothetical protein	-
GR153_RS13735 (GR153_013745)	3301379..3301837	+	459	WP_001239009.1	GNAT family N-acetyltransferase	-
GR153_RS13740 (GR153_013750)	3302053..3302913	-	861	WP_000682239.1	hypothetical protein	-
GR153_RS13745 (GR153_013755)	3303424..3304182	-	759	WP_000636956.1	DUF1554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15139.76 Da
• Isoelectric Point: 9.6893

>T208729 WP_000281042.1 NZ_CP077942:3299111-3299509 [Leptospira interrogans serovar Bataviae]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Nucleotide

• Length: 399 bp

>T208729 NZ_CP103718:994593-995006 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGCTTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATCAAGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8741.03 Da
• Isoelectric Point: 8.6588

>AT208729 WP_001192479.1 NZ_CP077942:3298881-3299111 [Leptospira interrogans serovar Bataviae]
MQTAKLFINGRSQAVRLPKEFQFAGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Nucleotide

• Length: 231 bp

>AT208729 NZ_CP103718:994346-994612 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTCAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAATTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0F6HAN6