Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 2882688..2883264 Replicon chromosome
Accession NZ_CP077942
Organism Leptospira interrogans serovar Bataviae strain D64

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag GR153_RS12080 Protein ID WP_000617906.1
Coordinates 2882688..2883029 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag GR153_RS12085 Protein ID WP_000844758.1
Coordinates 2883016..2883264 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GR153_RS12060 (GR153_012070) 2877935..2878339 - 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -
GR153_RS12065 (GR153_012075) 2878336..2878803 - 468 WP_000060053.1 hypothetical protein -
GR153_RS12070 (GR153_012080) 2879607..2880542 + 936 WP_000004530.1 ornithine carbamoyltransferase -
GR153_RS12075 (GR153_012085) 2880682..2881674 + 993 WP_000041092.1 aldolase/citrate lyase family protein -
GR153_RS12080 (GR153_012090) 2882688..2883029 - 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GR153_RS12085 (GR153_012095) 2883016..2883264 - 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
GR153_RS12090 (GR153_012100) 2884081..2884377 - 297 WP_000477061.1 helix-turn-helix transcriptional regulator -
GR153_RS12095 (GR153_012105) 2884531..2884972 + 442 Protein_2404 hypothetical protein -
GR153_RS12100 (GR153_012110) 2885606..2887588 - 1983 WP_000802799.1 HD family phosphohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T208728 WP_000617906.1 NZ_CP077942:c2883029-2882688 [Leptospira interrogans serovar Bataviae]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T208728 NZ_CP103718:c837641-837264 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT208728 WP_000844758.1 NZ_CP077942:c2883264-2883016 [Leptospira interrogans serovar Bataviae]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT208728 NZ_CP103718:c838098-837718 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGTTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References