Detailed information of TA system TA208718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2218680..2219209
• Replicon: chromosome
• Accession: NZ_CP077936
• Organism: Staphylococcus aureus strain 319

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU496_RS11180
• Protein ID: WP_225798545.1
• Coordinates: 2218680..2219042 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU496_RS11185
• Protein ID: WP_000948331.1
• Coordinates: 2219039..2219209 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU496_RS11160 (2215659)	2215659..2216429	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
KU496_RS11165 (2216404)	2216404..2216883	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU496_RS11170 (2216885)	2216885..2217211	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU496_RS11175 (2217330)	2217330..2218331	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU496_RS11180 (2218680)	2218680..2219042	-	363	WP_225798545.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU496_RS11185 (2219039)	2219039..2219209	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU496_RS11190 (2219294)	2219294..2220442	-	1149	WP_001281140.1	alanine racemase	-
KU496_RS11195 (2220508)	2220508..2220867	-	360	WP_000581197.1	holo-ACP synthase	-
KU496_RS11200 (2220871)	2220871..2221362	-	492	WP_001286801.1	PH domain-containing protein	-
KU496_RS11205 (2221355)	2221355..2222932	-	1578	WP_001294646.1	PH domain-containing protein	-
KU496_RS11210 (2222925)	2222925..2223404	-	480	WP_001287082.1	hypothetical protein	-
KU496_RS11215 (2223613)	2223613..2224173	-	561	WP_001092414.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13468.76 Da
• Isoelectric Point: 10.2892

>T208718 WP_225798545.1 NZ_CP077936:c2219042-2218680 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDNKMKEVDNALMISLGLNAVVHQKN

Nucleotide

• Length: 363 bp

>T208718 NZ_CP103714:c4591962-4591429 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208718 WP_000948331.1 NZ_CP077936:c2219209-2219039 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208718 NZ_CP103714:c4592239-4591973 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1