Detailed information of TA system TA208707

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 90316..90845
• Replicon: plasmid unnamed2
• Accession: NZ_CP077935
• Organism: Staphylococcus aureus strain 359

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU512_RS13690
• Protein ID: WP_000621175.1
• Coordinates: 90483..90845 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU512_RS13685
• Protein ID: WP_000948331.1
• Coordinates: 90316..90486 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU512_RS13655 (KU512_13635)	85352..85912	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
KU512_RS13660 (KU512_13640)	86121..86600	+	480	WP_001287088.1	hypothetical protein	-
KU512_RS13665 (KU512_13645)	86593..88176	+	1584	WP_001294626.1	PH domain-containing protein	-
KU512_RS13670 (KU512_13650)	88163..88654	+	492	WP_001205910.1	PH domain-containing protein	-
KU512_RS13675 (KU512_13655)	88658..89017	+	360	WP_000581200.1	holo-ACP synthase	-
KU512_RS13680 (KU512_13660)	89083..90231	+	1149	WP_001281145.1	alanine racemase	-
KU512_RS13685 (KU512_13665)	90316..90486	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU512_RS13690 (KU512_13670)	90483..90845	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU512_RS13695 (KU512_13675)	91194..92195	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KU512_RS13700 (KU512_13680)	92314..92640	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KU512_RS13705 (KU512_13685)	92642..93121	+	480	WP_225806155.1	anti-sigma B factor RsbW	-
KU512_RS13710 (KU512_13690)	93096..93866	+	771	WP_225806156.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaZ	hld / groEL / hlb / map	1..256180	256180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208707 WP_000621175.1 NZ_CP077935:90483-90845 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208707 NZ_CP103711:130184-130534 [Escherichia coli O25b:H4-ST131]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208707 WP_000948331.1 NZ_CP077935:90316-90486 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208707 NZ_CP103711:130539-130841 [Escherichia coli O25b:H4-ST131]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1