Detailed information of TA system TA208706

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 70574..71088
• Replicon: plasmid unnamed2
• Accession: NZ_CP077935
• Organism: Staphylococcus aureus strain 359

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: KU512_RS13600
• Protein ID: WP_000581792.1
• Coordinates: 70879..71088 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: KU512_RS13595
• Protein ID: WP_000138932.1
• Coordinates: 70574..70867 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU512_RS13575 (KU512_13555)	65837..66478	+	642	WP_000483153.1	thiamine phosphate synthase	-
KU512_RS13580 (KU512_13560)	66564..67436	+	873	WP_000725802.1	membrane protein insertase YidC	-
KU512_RS13585 (KU512_13565)	68249..68896	-	648	WP_001187629.1	HD domain-containing protein	-
KU512_RS13590 (KU512_13570)	68924..70408	-	1485	WP_050956558.1	cardiolipin synthase	-
KU512_RS13595 (KU512_13575)	70574..70867	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
KU512_RS13600 (KU512_13580)	70879..71088	+	210	WP_000581792.1	heavy metal-associated domain-containing protein	Toxin
KU512_RS13605 (KU512_13585)	71216..71353	+	138	WP_000828354.1	Lmo0850 family protein	-
KU512_RS13610 (KU512_13590)	71617..72819	-	1203	WP_001109939.1	FtsW/RodA/SpoVE family cell cycle protein	-
KU512_RS13615 (KU512_13595)	73137..74207	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
KU512_RS13620 (KU512_13600)	74222..75580	+	1359	WP_000611465.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaZ	hld / groEL / hlb / map	1..256180	256180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T208706 WP_000581792.1 NZ_CP077935:70879-71088 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T208706 NZ_CP103710:c4709067-4708756 [Escherichia coli O25b:H4-ST131]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT208706 WP_000138932.1 NZ_CP077935:70574-70867 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT208706 NZ_CP103710:c4708755-4708465 [Escherichia coli O25b:H4-ST131]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13