Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 70574..71088 Replicon plasmid unnamed2
Accession NZ_CP077935
Organism Staphylococcus aureus strain 359

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag KU512_RS13600 Protein ID WP_000581792.1
Coordinates 70879..71088 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag KU512_RS13595 Protein ID WP_000138932.1
Coordinates 70574..70867 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU512_RS13575 (KU512_13555) 65837..66478 + 642 WP_000483153.1 thiamine phosphate synthase -
KU512_RS13580 (KU512_13560) 66564..67436 + 873 WP_000725802.1 membrane protein insertase YidC -
KU512_RS13585 (KU512_13565) 68249..68896 - 648 WP_001187629.1 HD domain-containing protein -
KU512_RS13590 (KU512_13570) 68924..70408 - 1485 WP_050956558.1 cardiolipin synthase -
KU512_RS13595 (KU512_13575) 70574..70867 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
KU512_RS13600 (KU512_13580) 70879..71088 + 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
KU512_RS13605 (KU512_13585) 71216..71353 + 138 WP_000828354.1 Lmo0850 family protein -
KU512_RS13610 (KU512_13590) 71617..72819 - 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
KU512_RS13615 (KU512_13595) 73137..74207 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
KU512_RS13620 (KU512_13600) 74222..75580 + 1359 WP_000611465.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaZ hld / groEL / hlb / map 1..256180 256180


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T208706 WP_000581792.1 NZ_CP077935:70879-71088 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T208706 NZ_CP103710:c4709067-4708756 [Escherichia coli O25b:H4-ST131]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT208706 WP_000138932.1 NZ_CP077935:70574-70867 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT208706 NZ_CP103710:c4708755-4708465 [Escherichia coli O25b:H4-ST131]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References