Detailed information of TA system TA208689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1552662..1553191
• Replicon: chromosome
• Accession: NZ_CP077932
• Organism: Staphylococcus aureus strain 194

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KU515_RS07840
• Protein ID: WP_000621175.1
• Coordinates: 1552662..1553024 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KU515_RS07845
• Protein ID: WP_000948331.1
• Coordinates: 1553021..1553191 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU515_RS07820 (1549641)	1549641..1550411	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
KU515_RS07825 (1550386)	1550386..1550865	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
KU515_RS07830 (1550867)	1550867..1551193	-	327	WP_001052491.1	anti-sigma factor antagonist	-
KU515_RS07835 (1551312)	1551312..1552313	-	1002	WP_031928242.1	PP2C family protein-serine/threonine phosphatase	-
KU515_RS07840 (1552662)	1552662..1553024	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KU515_RS07845 (1553021)	1553021..1553191	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KU515_RS07850 (1553276)	1553276..1554424	-	1149	WP_001281140.1	alanine racemase	-
KU515_RS07855 (1554490)	1554490..1554849	-	360	WP_000581199.1	holo-ACP synthase	-
KU515_RS07860 (1554853)	1554853..1555344	-	492	WP_001205907.1	PH domain-containing protein	-
KU515_RS07865 (1555331)	1555331..1556914	-	1584	WP_001294637.1	PH domain-containing protein	-
KU515_RS07870 (1556907)	1556907..1557386	-	480	WP_001287077.1	hypothetical protein	-
KU515_RS07875 (1557594)	1557594..1558154	-	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T208689 WP_000621175.1 NZ_CP077932:c1553024-1552662 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T208689 NZ_CP103710:287747-288274 [Escherichia coli O25b:H4-ST131]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT208689 WP_000948331.1 NZ_CP077932:c1553191-1553021 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT208689 NZ_CP103710:287474-287740 [Escherichia coli O25b:H4-ST131]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1